栽培绿豆V1128抗豆象基因定位

刘长友,苏秋竹,范保杰,曹志敏,张志肖,武晶,程须珍,田静

Genetic Mapping of Bruchid Resistance Gene in Mungbean V1128

Chang-You LIU,Qiu-Zhu SU,Bao-Jie FAN,Zhi-Min CAO,Zhi-Xiao ZHANG,Jing WU,Xu-Zhen CHENG,Jing TIAN

表1 多态性标记信息

Table 1 Information of polymorphic DNA markers used in this study

标记名称 Marker 标记类型 Type 引物序列 Primer sequence (5′-3′) 退火温度 Tm 文献来源 Reference Mchr5-17 SSR F: GCTTGCTTATGCTCAAAACT; R: TACAGATAAACCCAAGCCAT 55 [23] Mchr5-20 SSR F: TCAAAACTTTCACTGGACCT; R: GCTGTTTGTCACATGCATAA 55 [23] Mchr5-23 SSR F: ATCTTAATCCCATCCTTGGT; R: AACTGGCTTGTAAGGTGAGA 55 [23] Mchr5-25 SSR F: CCGTTGTGAATCAACTTTTC; R: GAGTCGTCGTGTAATCCTTC 55 [23] Mchr5-32 SSR F: ACTTGTAGGTGGAAGAGATGA; R: GATTAAGGGCGTGTTTTGT 55 [23] Mchr5-33 SSR F: CTAATGAAACAGGACAAGGG; R: CTCACTCTTCTCATTCCACC 55 [23] Mchr5-36 SSR F: ACCTACTGATTGGTGTTTGG; R: CAGTGAATGCTGACAGTGAC 55 [23] Mchr5-37 SSR F: AACGGTTGGAGTTAGGAAAT; R: TGAACATCACCAACTATCAC 55 [23] Mchr3-85 SSR F: AACAGCGTTGATTTATGGAC; R: AATCATGTTGGTGTGTTGTG 55 [23] Mchr3-86 SSR F: TGCCTAAGGGTCAATTTCTA; R: ATGCACCAGAAGACAAAAAC 55 [23] Mchr3-87 SSR F: AATGAGGTAATGCAGAGGTG; R: GACAAGGGTTGTTGTTCACT 55 [23] Mchr3-89 SSR F: GAATTAAAGCCCTTGTTCTG; R: GGTGAATTTTCTGTTTCCAC 55 [23] Mchr3-96 SSR F: CTGATGCTATTTCCATCCAT; R: TAACCTTTTGCATTTGGTGC 55 [23] MUS150 SSR F: GCTGTTTGTCACATGCATAA; R: TCAAAACTTTCACTGGACCT 55 [23] MUS365 SSR F: TGAGCCCGATTTTTATCTC;R: GCTCACAGATAACTGACACAAC 55 [23] MUS569 SSR F: GGAGGGGATTTTTAAGATTG; R: GGATACGATTTTGTCGTGTT 55 [23] HAAS_VR_379 SSR F: CCTATCCGAATCGACACCAC; R: GTAGCAATAGCAGCCCAAGG 55 [22] HAAS_VR_106 SSR F: ACGGCTATTCATCGTTTTGC; R: CAACCCGAAGCCAAAAACTA 55 [22] HAAS_VR_89 SSR F: GCTGGAAGGATCCAATTTCA; R: TCGCCATTCCCAAGATAAAG 55 [22] HAAS_VR_1701 SSR F: CCGGGGTGAAATTGATACAC; R: CAAAGGGGCTATGAACAGGA 55 [22] VRID1 Indel F: TCGGTTTCAGCTCGATAGATTC; R: GATGTTGTCTGAAGTAGTGGTA 55 [21] VRID5 Indel F: AGAATAAAATGAATCTAGAAGACCA; R: TGAATTAATTTTCTTACCCTTGT 50 [21] VRBR-SSR016 SSR F: GACGGCTAGGTACAACACTGC; R: TTTAGAGCAATTGGGTGGATTT 55 [20] VRBR-SSR024 SSR F: TTTTGTGGACACTCCTTCCA; R: AAGCGTCACACCCTCAATTC 55 [20] VRBR-SSR030 SSR F: CAGCTAGGAAACTCACCAAACC; R: CAGCTGGCAGGTGAAATATG 55 [20] VRBR-SSR032 SSR F: GGTTATTTTGATCTAAAGGGCCA; R: GTGTGAGAAGATTTGGGAATGTAA 55 [20] VRBR-SSR033 SSR F: CTCAAGTCTTATGTTTCCCCCTAT; R: GCACTAAAGGACTTTCCTTGAAC 55 [20] VRBR-SSR039 SSR F: AAGTTGGTGTAGCACTTGCAGA; R: AATGAATTAAAAGAAAACACTACTG 55 [20] GBssr-MB87 SSR F: TCCCTTGTGGGAGATCCT; R: CTTTGCCACACTCCTTGC 55 [24] DMB158 SSR F: TGGAAAATTTGCAGCAGTTG; R: ATTGATGGAGGGCGGAAGTA 55 [25] PVBR201 SSR F: GTGATGGTGCTGCTTTTCAA; R: ATGCGTGGGGAGAAGTAAGA 50 [26]