白菜型冬油菜RuBisCo蛋白亚基基因rbcLrbcS的克隆及其在干旱胁迫下的表达
米超,赵艳宁,刘自刚,陈其鲜,孙万仓,方彦,李学才,武军艳

Cloning of RuBisCo Subunits Genes rbcL and rbcS from Winter Rapeseed (Brassica rapa) and Their Expression under Drought Stress
Chao MI,Yan-Ning ZHAO,Zi-Gang LIU,Qi-Xian CHEN,Wan-Cang SUN,Yan FANG,Xue-Cai LI,Jun-Yan WU
表1 LC-MS鉴定结果
Table 1 Identification of special proteins by LC-MS
序号
Spot No.
登录号
Accession
蛋白名称
Protein name
分子量/等电点
MW/pI
得分
Score
来源
Resource
上调/下调
Up/down
1 gi|541620 低温相关蛋白BN115
Low-temperature regulated protein BN115
14.8 kDa/7.75 81.8 甘蓝型油菜
Brassica napus
+10
2 gi|229893642 病毒抗性蛋白
Virus-resistance protein
20.1 kDa/7.31 46.1 大白菜
Brassica rapa subsp. chinensis
+20
3 gi|109389998 叶绿体叶绿素a/b结合蛋白
Chloroplast chlorophyll a/b binding protein
28.3 kDa/5.22 46.2 甘蓝型油菜
Brassica napus
+12
4 gi|132091 叶绿体Rubisco F1小链
Ribulose bisphosphate carboxylase small
chain F1, chloroplastic; Short
20.2 kDa/7.31 111 甘蓝型油菜
Brassica napus
-10
5 gi|171702843 半胱氨酸蛋白酶
Cysteine protease
50.5 kDa/5.17 73.7 白菜型油菜
Brassica rapa
+18
6 gi|19570344 核苷二磷酸激酶1
Nucleoside diphosphate kinase 1
16.4 kDa/6.05 101 白菜型油菜
Brassica rapa
-11
7 gi|15225120 应激蛋白α-β结构域
Stress responsive alpha-beta barrel domain
protein
22.5 kDa/5.04 135 拟南芥
Arabidopsis thaliana
+13
8 gi|15241956 谷胱甘肽S-转移酶家族蛋白
Glutathione S-transferase family protein
31.9 kDa/8.03 79.1 拟南芥
Arabidopsis thaliana
-15
序号
Spot No.
登录号
Accession
蛋白名称
Protein name
分子量/等电点
MW/pI
得分
Score
来源
Resource
上调/下调
Up/down
9 gi|356494238 磷酸甘露糖异构酶
Phosphomannomutase
27.9 kDa/5.23 95.6 大白菜
Brassica rapa subsp. chinensis
+13
10 gi|29778743| 叶绿体β-碳酸酐酶
Chloroplast beta-carbonic anhydrase
37.7kDa/6.00 106 甘蓝型油菜
Brassica napus
+11
11 gi|14009294 假定的6-磷酸葡萄糖酸内酯酶
Putative 6-phosphogluconolactonase
29.3 kDa/5.90 114 埃塞俄比亚芥
Brassica carinata
+14
12 gi|15234962 DNAJ热激蛋白N-端结构域
DNAJ heat shock N-terminal
domain-containing protein
38.4 kDa/7.38 50.8 拟南芥
Arabidopsis thaliana
+12
13 gi|211905345 上皮硫特异蛋白
Epithiospecifier protein
37.9kDa/5.71 73.8 白菜
Brassica rapa subsp. pekinensis
+15
14 gi|62321480 脂氧合酶
Lipoxygenase
103.3 kDa/5.36 96.4 拟南芥
Arabidopsis thaliana
-14
15 gi|89257686 假定的乙酰鸟苷酸脱乙酰基酶
Acetylornithine deacetylase, putative
48.0 kDa/5.62 129.0 甘蓝
Brassica oleracea
+12
16 gi|19553697| β-1,3-葡聚糖酶
Beta-1,3-glucanase
38.9 kDa/4.89 95.6 大白菜
Brassica rapa subsp. chinensis
+16
17 gi|356494248 L-半乳糖脱氢酶
L-galactose dehydrogenase
34.9 kDa/5.08 68 大白菜
Brassica rapa subsp. chinensis
-9
18 gi|41584275 热休克同源蛋白70
Heat shock cognate protein 70
28.1 kDa/5.17 65.7 山嵛菜
Eutrema halophilum
-16
19 gi|28540855 可溶性淀粉合成酶
Soluble starch synthase
71.6 kDa/5.24 103 白菜
Brassica rapa subsp. pekinensis
+17
20 gi|15229519 二羟丙酮激酶
Dihydroxyacetone kinase
61.9 kDa/5.22 111 拟南芥
Arabidopsis thaliana
-11
21 gi|197245081 玉米黄质环氧酶
Zeaxanthin epoxidase
79.8 kDa/5.67 108 甘蓝型油菜
Brassica napus
-13