甘蓝型油菜蔗糖磷酸合酶(SPS)基因家族成员鉴定及表达分析

张莉, 荐红举, 杨博, 张翱翔, 张超, 杨鸿, 张立源, 刘列钊, 徐新福, 卢坤, 李加纳

Genome-wide Analysis and Expression Profiling of SPS Gene Family in Brassica nupus L.

Li ZHANG, Hong-Ju JIAN, Bo YANG, Ao-Xiang ZHANG, Chao ZHANG, Hong YANG, Li-Yuan ZHANG, Lie-Zhao LIU, Xin-Fu XU, Kun LU, Jia-Na LI

附表2 引物序列

Supplementary table 2 Primers of sequences

基因 Gene 引物序列 Primer sequence (5′-3′) Actin7 F: TGGGTTTGCTGGTGACGAT; R: TGCCTAGGACGACCAACAATACT BnSPSA1-1 F: AGGATAAGTATATCCCGAAGGAACTC; R: CGTCACCAGCATCAGCGTAGT BnSPSA1-2 F: CACATGATGGTGATATGGAAGACG; R: GATATTCTTTTTTGGGTCGGGC BnSPSA1-3 F: GAGACTGATCTTCACAAGTCATGGA; R: TCCTTTTCCTCATGCTGCTTCT BnSPSA2-1 F: AAATGGCAGAGAGTCGAGTTCG; R: AGCTTAGCTTTTCTTTCAGCGGT BnSPSA2-2 F: TGCTAGTGCGATGGGGGAT; R: GGTAGCTTCTGTTGCCGTGC BnSPSA2-3 F: TGACTGGAGCTATGCCGAACCT; R: GGACATCTGCATCACATGGCTAAG BnSPSA2-4 F: CGGTTTGATACGAGGTGAGAACAT; R: GCATCTCAGACGGTTCAGCG BnSPSB-1 F: ATGTCGTATGAGACATCCCCAGT; R: TATTCGGTTCAAGAGACGCATTC BnSPSB-2 F: CGAGGAACTTATCTCTGGCACAC; R: CCAATAAAAGGACTCTCTGACGG BnSPSC-1 F: ACGTGAAAAAGGACGCAATGAT; R: GCAGATAGAGGCAGGCACAATG BnSPSC-2 F: GTGATTACTGCTGATTCCTACGATG; R: TATTTTTCCCTTGCCCGATGT