基于棉花U6启动子的海岛棉CRISPR/Cas9基因组编辑体系的建立

李继洋, 雷建峰, 代培红, 姚瑞, 曲延英, 陈全家, 李月, 刘晓东

Establishment of CRISPR/Cas9 Genome Editing System Based on GbU6 Promoters in Cotton (Gossypium barbadense L.)

Ji-Yang LI, Jian-Feng LEI, Pei-Hong DAI, Rui YAO, Yan-Ying QU, Quan-Jia CHEN, Yue LI, Xiao-Dong LIU

表1 本研究使用的引物信息

Table 1 Primers used in this study

引物名称 Primer name 序列 Sequence (5'-3') 引物名称 Primer name 序列 Sequence (5'-3') GbU6-4PERA1-sg1F GATTGTTCGCAGAATGCATGACGG GbU6-5PGGB-sg2F AAGTGCTCTGTCGAAGTACTGAAG GbU6-5PERA1-sg1F AAGTGTTCGCAGAATGCATGACGG GbU6GGB-sg2R AAACCTTCAGTACTTCGACAGAGC GbU6ERA1-sg1R AAACCCGTCATGCATTCTGCGAAC Test GGB-sgF AAGTGGAAAGAGAATGGCGAC GbU6-4PERA1-sg2F GATTGTCTTTCGCAGAATGCATGA Test GGB-sgR AGCTAATTCGCTATTGCAATCAATC GbU6-5PERA1-sg2F AAGTGTCTTTCGCAGAATGCATGA Test ERA1-sgF GTATCCTCTTACCGCCTTTC GbU6ERA1-sg2R AAACTCATGCATTCTGCGAAAGAC Test ERA1-sgF CATACTTACTGAGATGGCTGT GbU6-4PGGB-sg1F GATTGTGTCGAAGTACTGAAGCGG FJ-1F GTAAAACGACGGCCAG GbU6-5PGGB-sg1F AAGTGTGTCGAAGTACTGAAGCGG FJ-1R CAGGAAACAGCTATGAC GbU6GGB-sg1R AAACCCGCTTCAGTACTTCGACAC FJ-2F TAAACTGAAGGCGGGAAACG GbU6-4PGGB-sg2F GATTGCTCTGTCGAAGTACTGAAG FJ-2F CGGTTCTGTCAGTTCCAAACG