甘蓝型油菜BnGS3和BnGhd7的同源克隆及其与油菜产量相关性状的关系

薛志飞, 王夏, 李付鹏, 马朝芝

Homologous Cloning of BnGS3 and BnGhd7 Genes in Brassica napus and Their Relationship with Rapeseed Yield-related Traits

Zhi-Fei XUE, Xia WANG, Fu-Peng LI, Chao-Zhi MA

附表1 同源克隆BnGS3和BnGhd7的引物

Supplementary table 1 Primers used in homologous clonging of BnGS3 and BnGhd7

引物 Primer 正向序列 Forward sequence (5'-3') 反向序列 Reverse sequence (5'-3') 退火温度 Tm (°C) bngs-1-1 TACTCATCCTCCTCCTCC CGCTCATCATCGAGAACT 55 bngs-1-2 GCGTAATGCCTCAGCTAC GACCCATCATCATCGAGA 55 bngs-1-3 GCATGATGCTTTCACCAC CGATCATCATCGAGAACT 55 gs3-1 TGTTAGGGTTTCTGTTGGTGG CCAGATGCTGCAAAGAGTAAG 59 gs3-2 GGGTTTCTGTTGGTGGGCTT CCCATGAGGTATGTCAGCAT 59 gs3-3 TTCTAGACAATGAATGGCAGG ACGAAAGAGTCGACGACACTG 58 gs3-4 TTCTAGACAATGAATGGCAG TGCAACTGCAAGATCAAATG 59 gs3-5 CCGCAGCGGAAAAGGTATGT GTCCAGATGCTGCAAAGAGT 59 ghd7-2-4 CCAAAAGCCAACGTCACCAT GCGATCAGCGACCATTAAAG 56 ghd7-2-5 CCAAAAGCCAACGTCACCAT GGAGCGACCGAAAACTACAT 54 ghd7-2-6 TCCCTCACCAACAACAAACC ATCCTTGGTCTTTTCTCTGC 55 ghd7-1 CAAAAGCCAACGTCACCATC CGACCATTAAAGAACAGGCT 58 ghd7-2 TTCGGATTCGGTTCTGGTTC GCTCACATGATTGACAGACT 59 ghd7-3 AGCAGAGGCGGCTTCTTGGT GCGATCAGCGACCATTAAAG 59 ghd7-4 CCAAAAGCCAACGTCACCAT GCGATCAGCGACCATTAAAG 58 ghd7-5 CCAAAAGCCAACGTCACCAT GGAGCGACCGAAAACTACAT 59 ghd7-6 TCCCTCACCAACAACAAACC ATCCTTGGTCTTTTCTCTGC 59 ghd7-7 GGGTTGTTCCACTTCAGGTT ACCCATGGAAAGTGGTAGAT 59 ghd7-8 CAAAAGCCAACGTCACCATC CATCCGATATTTTTGTCTCC 60 ghd7-9 GATCCATCACGGGCCATAAC GGGTTTGAAACTGTTGTCTC 59