基于田间表型和Bru1基因检测分析甘蔗褐锈病抗性遗传

李竹, 许莉萍, 苏亚春, 吴期滨, 成伟, 孙婷婷, 高世武

Analysis of Brown Rust Resistance Inheritance Based on Field Phenotypes and Detection of Bru1 Gene in Sugarcane

Zhu LI, Li-Ping XU, Ya-Chun SU, Qi-Bin WU, Wei CHENG, Ting-Ting SUN, Shi-Wu GAO

表1 甘蔗褐锈病抗病性鉴定评价标准

Table 1 Identification standard of sugarcane resistance to brown rust disease

分级标准 Grade 抗病性 Resistance 叶片侵染状况 Leaf infection performance 1 高抗 High resistance 无症状。 No symptoms. 2 抗病 Resistance 有坏死斑, 病斑占叶面积10%以下。 The area of necrotic spots on leaves is lower than 10%. 3 中抗 Moderate resistance 植株上有一些孢子堆, 病斑占叶面积11%~25%。 There are piles of spores visible on the plant. The percentages of lesion spots’ area to leaf area are 11%-25%. 4 中感 Moderate susceptibility 上层1~3片叶有一些孢子堆, 同时下层叶有许多孢子堆, 病斑占叶面积26%~35%。 There are some spore piles from leaf 1 to leaf 3 at the top and many spores on lower leaves. The percentages of lesion spots’ area in leaf are 26%-35%. 5 感病1 Susceptibility 1 上层1~3片叶有极多孢子堆, 同时下层叶有轻微的坏死, 病斑占叶面积36%~50%。 There are abundant spore piles from leaf 1 to leaf 3 at the top and slight necrotic spots on lower leaves. The percentages of lesion spots’ area in leaf are 36%-50%. 6 感病2 Susceptibility 2 上层1~3片叶有极多孢子堆且下层叶有比第5级更多的坏死, 病斑占叶面积51%~60%。 There are abundant spore piles from leaf 1 to leaf 3 at the top and more necrotic spots on lower leaves than those at grade 5. The percentages of lesion spots’ area in leaf are 51%-60%. 7 感病3 Susceptibility 3 上层1~3片叶有极多孢子堆, 下层叶坏死, 病斑占叶面积61%~75%。 There are abundant spore piles from leaf 1 to leaf 3 at the top and necrotic spots on lower leaves. The percentages of lesion spots’ area in leaf are 61%-75%. 8 高感1 High susceptibility 1 上层1~3片叶有某些坏死, 病斑占叶面积76%~90%。 There are some necrotic spots from leaf 1 to leaf 3 at the top. The percentages of lesion spots’ area in leaf are 76%-90%. 9 高感2 High susceptibility 2 叶片坏死, 植株濒于死亡, 病斑占叶面积91%~100%。 Leaves are necrotic and plants are almost dead. The percentages of lesion spots’ area in leaf are 91%-100%.