应用SNP精准鉴定大豆种质及构建可扫描身份证

魏中艳, 李慧慧, 李骏, YasirA.Gamar, 马岩松, 邱丽娟

Accurate Identification of Varieties by Nucleotide Polymorphisms and Establishment of Scannable Variety IDs for Soybean Germplasm

Zhong-Yan WEI, Hui-Hui LI, Jun LI, A. Gamar Yasir, Yan-Song MA, Li-Juan QIU

表1 SNP标记多样性分析

Table 1 Analysis of SNP markers diversity

性状 Trait 基因 Gene SNP位点 SNP locus 染色体 Chr. 物理位置 Physical location 纯合等位 基因比例 Homozygous allele ratio (%) 变异类型 Mutation type 基因型 Genotype 遗传多样性指数 Genetic diversity index 结荚习性 Stem growth habit Glyma19g37890 AR2 19 44981190 89.60 1TV G/T 0.334 AR4 19 44980194 95.14 1TV G/A 0.194 AR5 19 44980087 89.95 2TS A/T 0.351 茸毛色 Pubescence color Glyma06g21920 AR7 6 18540661 87.25 3D A/- 0.382 TT8 6 18540852 57.56 3D C/- 0.701 熟期 Maturity Glyma06g23026 TT10 6 20007177 99.16 3D A/- 0.049 Glyma10g36600 TT11 10 44732850 62.39 1TV A/T 0.696 Glyma19g41210 TT12 19 47516339 99.83 2TS G/A 0.012 TT13 19 47513779 99.16 3D -/T 0.048 Glyma20g22160 TT14 20 32089731 100.00 4ND G/- 0 PRO15 20 32091662 99.33 3D A/- 0.040 PRO16 20 32091044 100.00 4ND T/- 0 胞囊线虫 SCN Glyma18g02681 PRO19 18 1712103 55.54 2TS T/C 0.722 Glyma08g11350 AT21 8 8280937 90.12 2TS T/C 0.360 AT22 8 8281297 88.57 1TV A/T 0.337 AT23 8 8281564 94.59 2TS A/G 0.376 花叶病毒病 SMV Glyma02g13600 AT26 2 11929770 99.50 2TS A/G 0.210 SER27 2 11930414 65.28 1TV G/T 0.032 Glyma02g13380 SER32 2 11693604 88.01 1TV C/G 0.665 Glyma13g26000 SER36 13 29227216 90.12 1TV G/C 0.439 Glyma14g38500 SER43 14 47631542 57.94 1TV T/A 0.708 SER46 14 47633021 100.00 4ND G/C 0 百粒重 100-seed weight Glyma13g22850 SER64 13 27548370 80.57 1TV A/C 0.514