玉米油菜素甾醇生物合成关键酶基因ZmCYP90B1的克隆及其对逆境胁迫的响应

段方猛, 罗秋兰, 鲁雪莉, 齐娜伟, 刘宪舜, 宋雯雯

Cloning of the Key Gene ZmCYP90B1 in Brassinosteroids Biosynthesis from Zea mays and Its Response to Adversity Stresses

Fang-Meng DUAN, Qiu-Lan LUO, Xue-Li LU, Na-Wei QI, Xian-Shun LIU, Wen-Wen SONG

表1 克隆基因所用引物

Table 1 Primers used in cloning gene

引物 Primer 序列 Sequence (5°-3°) DP-F GGCGGCATCCTGGGCAAGT DP-R CCCGCGAAGAGCAGGCTCA 5°-GSP TGGACCACTTGCCCAGGATGCC 3°-GSP GAAGATGGAGGACAGGCTTGAGA FP-F CTCTCTCCAACCCATCCGTC FP-R GGGAGGTAATGTTGGGGTTTG