玉米油菜素甾醇生物合成关键酶基因ZmCYP90B1的克隆及其对逆境胁迫的响应
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段方猛, 罗秋兰, 鲁雪莉, 齐娜伟, 刘宪舜, 宋雯雯
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Cloning of the Key Gene ZmCYP90B1 in Brassinosteroids Biosynthesis from Zea mays and Its Response to Adversity Stresses
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Fang-Meng DUAN, Qiu-Lan LUO, Xue-Li LU, Na-Wei QI, Xian-Shun LIU, Wen-Wen SONG
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| 表2 荧光定量PCR所需引物信息 |
| Table 2 Specific primers used for real-time PCR analysis |
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基因名称
Gene name | GenBank登录号
GenBank number | 引物序列
Primer sequence (5°-3°) | 产物长度
Product size
(bp) | 退火温度
Annealing
temperature (°C) | | NtAPX | NM_001324874.1 | F: CAAATGTAAGAGGAAACTCAGAGGA
R: AGCAACAACTCCAGCTAATTGATAG | 262 | 60 | | NtCAT | NM_001326196.1 | F: AGGTACCGCTCATTCACACC
R: AAGCAAGCTTTTGACCCAGA | 151 | 60 | | NtSOD | AF443178.1 | F: CCGTCGCCAAATTGCATAG
R: CGATAGCCCAACCAAGAGAAC | 238 | 62 | | NtNCED1 | NM_001326185.1 | F: CTATTTCCACTTCAAAACCAACCAC
R: GGCACTTTCCACGGCATCT | 131 | 62 | | NtERD10C | AB049337.1 | F: ACGTGGAGGCTACAGATCGTGGTTT
R: TCTCCACTGGTACAGCCGTGTCCTCAC | 366 | 70 | | NtLEA5 | AF053076.1 | F: GAACCCAACAAGAGCGAGAGA
R: CGACAGGAAGCATTGACGAG | 350 | 62 | | Tob103 | U60495.1 | F: CAAGGAAATCACCGCTTTGG
R: AAGGGATGCGAGGATGGA | 106 | 60 | | ZmCYP90B1 | KY242373 | F: GGCTGTGGTCTCGTGTTTTATG
R: ACCATCCTCCCCTTTGCTCT | 104 | 62 | | Actin | NM_00115473.1 | F: CTGAGGTTCTATTCCAGCCATCC
R: CCACCACTGAGGACAACATTACC | 133 | 62 |
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