玉米油菜素甾醇生物合成关键酶基因ZmCYP90B1的克隆及其对逆境胁迫的响应

段方猛, 罗秋兰, 鲁雪莉, 齐娜伟, 刘宪舜, 宋雯雯

Cloning of the Key Gene ZmCYP90B1 in Brassinosteroids Biosynthesis from Zea mays and Its Response to Adversity Stresses

Fang-Meng DUAN, Qiu-Lan LUO, Xue-Li LU, Na-Wei QI, Xian-Shun LIU, Wen-Wen SONG

表3 不同植物来源的CYP90B1氨基酸理化性质的比较

Table 3 Comparison of physical and chemical characteristics of amino acids of CYP90B1 from different plant species

类别 Category 物种 Species 氨基酸残基数 Residues 分子量 Molecular weight (kD) 等电点pI 负电荷氨基酸比例 Negatively charged residues (%) 正电荷氨基酸比例 Positively charged residues (%) 不稳定 系数 Instability index 总平均亲水性 Hydropathicity 单子叶植物 Monocotyledons Zea mays 506 57.66 9.54 10.47 13.44 46.74 -0.171 Dichanthelium oligosanthes 507 57.87 9.25 10.65 12.62 48.82 -0.161 Oryza brachyantha 504 57.12 9.27 10.52 12.70 52.69 -0.138 Aegilops tauschii 501 57.01 8.11 11.58 11.98 52.53 -0.133 Triticum urartu 525 59.75 8.09 11.62 12.00 51.61 -0.162 双子叶植物 Dicotyledons Arabidopsis thaliana 513 58.87 6.62 12.87 12.28 49.50 -0.301 Nicotiana attenuata 491 55.78 8.89 9.98 11.41 44.36 -0.166 Brassica napus 495 56.71 8.17 11.72 12.12 48.90 -0.213 Glycine soja 492 55.77 8.85 10.16 11.38 43.87 -0.128 Theobroma cacao 496 56.75 8.64 10.89 11.90 46.51 -0.219 Gossypium hirsutum 485 55.42 8.94 10.52 12.16 44.88 -0.163