一个新的玉米Vp15基因等位突变体的遗传分析与分子鉴定

王瑞, 张秀艳, 陈阳松, 杜依聪, 汤继华, 王国英, 郑军

Genetic Analysis and Molecular Characterization of a New Allelic Mutant of Vp15 Gene in Maize

Rui WANG, Xiu-Yan ZHANG, Yang-Song CHEN, Yi-Cong DU, Ji-Hua TANG, Guo-Ying WANG, Jun ZHENG

表1 本研究所用的引物

Table 1 Primers used in this study

引物 Primer 正向序列 Forward sequence (5°-3°) 反向序列 Reverse sequence (5°-3°) VP15-G-F1/R1 AAGGACGCGAGAGTTTTTGA GGTGAGCGAGTAACCCATGT VP15-G-F2/R2 ACATGGGTTACTCGCTCACC CTTCCTGGTAGCGAGACGTG VP15-G-F3/R3 GCTCGTTCACCATCCAGAAT AGCAAAGCCCTTTCTGAACA VP15-G-F4/R4 GGGCTTTGCTAAGATGTCTCC GGACAATACATAACTGCTCCCTAAA VP15-G-F5/R5 TCTTCCACTCTTCCTACCAGCTA AGGAGCCAACAACTGCTGAT VP15-G-F6/R6 GAACAATCGAGCCTTCTTCG TCGTCCTCCTTGACCACTTC VP15-G-F7/R7 CACCAACTGCAAGATCGAAA CCAACAGACGTCAACACCAG VP15-CDS-F1/R1 GGAACTCTCTGATACCTGGCTTT CAGTTCCGTGATTAGCCGC Zm-GAPDH-F/R CCCTTCATCACCACGGACTAC AACCTTCTTGGCACCACCCT