玉米胚乳母本印记基因ZmVIL1的克隆及印记特性分析
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刘朝显, 王久光, 梅秀鹏, 余婷婷, 王国强, 周练, 蔡一林
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Cloning and Imprinting Characterization Analyses of Paternally Expressed Gene ZmVIL1 in Maize Endosperm
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Chao-Xian LIU, Jiu-Guang WANG, Xiu-Peng MEI, Ting-Ting YU, Guo-Qiang WANG, Lian ZHOU, Yi-Lin CAI
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表1 ZmVIL1 5° RACE、3° RACE、CDS扩增及印记特性分析引物序列 |
Table 1 Primer sequences for 5° RACE, 3° RACE, CDS amplification and imprinting characterization analyses of ZmVIL1 |
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引物名称 Primer name | 正向引物序列 Forward primer sequence (5°-3°) | 反向引物序列 Reverse primer sequence (5°-3°) | 内切酶 Restriction enzyme | ZmVIL1-5° race | GCCCATCGCATTCCAACTGCCTTACTAC | | | ZmVIL1-5° race-nest-1 | GCGTTTAGGTCGGGGACAGAAGATGAG | | | ZmVIL1-5° race-nest-2 | GGTCAGGAGAACTCAACCACTCGTCAGC | | | ZmVIL1-3° race | CTCGGCAAGTAAGCAAGGTGAATAGCGT | | | ZmVIL1-3° race-nest-1 | GCAACTGGATGGGGATTATGAGCACTG | | | ZmVIL1-3° race-nest-2 | AGGTAGTAAGGCAGTTGGAATGCGATGG | | | ZmVIL1-CDS | ATGCCCAAAACACCTCCTGT | CTCAGCGAACCACAACAACAAA | | ZmVIL1-imprinting | ATTCAAAGAGCTGACGAGTGGT | TCATAATCCCCATCCAGTTGCC | Hph I |
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