玉米胚乳母本印记基因ZmVIL1的克隆及印记特性分析
刘朝显, 王久光, 梅秀鹏, 余婷婷, 王国强, 周练, 蔡一林

Cloning and Imprinting Characterization Analyses of Paternally Expressed Gene ZmVIL1 in Maize Endosperm
Chao-Xian LIU, Jiu-Guang WANG, Xiu-Peng MEI, Ting-Ting YU, Guo-Qiang WANG, Lian ZHOU, Yi-Lin CAI
表1 ZmVIL1 5° RACE、3° RACE、CDS扩增及印记特性分析引物序列
Table 1 Primer sequences for 5° RACE, 3° RACE, CDS amplification and imprinting characterization analyses of ZmVIL1
引物名称
Primer name
正向引物序列
Forward primer sequence (5°-3°)
反向引物序列
Reverse primer sequence (5°-3°)
内切酶
Restriction enzyme
ZmVIL1-5° race GCCCATCGCATTCCAACTGCCTTACTAC
ZmVIL1-5° race-nest-1 GCGTTTAGGTCGGGGACAGAAGATGAG
ZmVIL1-5° race-nest-2 GGTCAGGAGAACTCAACCACTCGTCAGC
ZmVIL1-3° race CTCGGCAAGTAAGCAAGGTGAATAGCGT
ZmVIL1-3° race-nest-1 GCAACTGGATGGGGATTATGAGCACTG
ZmVIL1-3° race-nest-2 AGGTAGTAAGGCAGTTGGAATGCGATGG
ZmVIL1-CDS ATGCCCAAAACACCTCCTGT CTCAGCGAACCACAACAACAAA
ZmVIL1-imprinting ATTCAAAGAGCTGACGAGTGGT TCATAATCCCCATCCAGTTGCC Hph I