玉米胚乳母本印记基因ZmVIL1的克隆及印记特性分析

刘朝显, 王久光, 梅秀鹏, 余婷婷, 王国强, 周练, 蔡一林

Cloning and Imprinting Characterization Analyses of Paternally Expressed Gene ZmVIL1 in Maize Endosperm

Chao-Xian LIU, Jiu-Guang WANG, Xiu-Peng MEI, Ting-Ting YU, Guo-Qiang WANG, Lian ZHOU, Yi-Lin CAI

表1 ZmVIL1 5° RACE、3° RACE、CDS扩增及印记特性分析引物序列

Table 1 Primer sequences for 5° RACE, 3° RACE, CDS amplification and imprinting characterization analyses of ZmVIL1

引物名称 Primer name 正向引物序列 Forward primer sequence (5°-3°) 反向引物序列 Reverse primer sequence (5°-3°) 内切酶 Restriction enzyme ZmVIL1-5° race GCCCATCGCATTCCAACTGCCTTACTAC ZmVIL1-5° race-nest-1 GCGTTTAGGTCGGGGACAGAAGATGAG ZmVIL1-5° race-nest-2 GGTCAGGAGAACTCAACCACTCGTCAGC ZmVIL1-3° race CTCGGCAAGTAAGCAAGGTGAATAGCGT ZmVIL1-3° race-nest-1 GCAACTGGATGGGGATTATGAGCACTG ZmVIL1-3° race-nest-2 AGGTAGTAAGGCAGTTGGAATGCGATGG ZmVIL1-CDS ATGCCCAAAACACCTCCTGT CTCAGCGAACCACAACAACAAA ZmVIL1-imprinting ATTCAAAGAGCTGACGAGTGGT TCATAATCCCCATCCAGTTGCC Hph I