甘蓝转录因子BoLH27的克隆与转基因甘蓝的表型分析
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梁云飞, 张林成, 蒲全明, 雷镇泽, 施松梅, 姜宇鹏, 任雪松, 高启国
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Cloning of BoLH27 Gene from Cabbage and Phenotype Analysis of Transgenic Cabbage
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Yun-Fei LIANG, Lin-Cheng ZHANG, Quan-Ming PU, Zhen-Ze LEI, Song-Mei SHI, Yu-Peng JIANG, Xue-Song REN, Qi-Guo GAO
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| 表1 试验使用的引物 |
| Table 1 Primers used in this study |
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引物名称及序列
Primer name and sequence (5°→3°) | 退火温度
Annealing temperature (°C) | klBoLH27S: ATGGAAGACCTCGAAGATGAGTACAAG
klBoLH27AS: GGTTTTTGGTACAATGAAACAAACTAGAAG | 63 | pcBoLH27S: GGATCCATGGAAGACCTCGAAGATGAGTACAAG
pcBoLH27AS: GGATCCGGTTTTTGGTACAATGAAACAAACTAGAAG
NOS: CCCGATCTAGTAACATAGATGACAC | 62 | jcBoLH27S: GGATCCATGGAAGACCTCGAAGATGAGTACAAG
jcBoLH27AS: GGATCCGGTTTTTGGTACAATGAAACAAACTAGAAG | 56.8 | qBoLH27S: AGATTAGAAGCAGAGATCCAAGAGC
qBoLH27AS: GAAGTATTGTAATCCATCTGCCTGAAC | 58 |
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