茶树叶片和胚根原生质体的分离及PEG诱导融合

彭章, 童华荣, 梁国鲁, 石艺琦, 袁连玉

Protoplast Isolation and Fusion Induced by PEG with Leaves and Roots of Tea Plant (Camellia sinensis L. O. Kuntze)

Zhang PENG, Hua-Rong TONG, Guo-Lu LIANG, Yi-Qi SHI, Lian-Yu YUAN

表1 不同酶解液组合对分离茶树嫩叶原生质体的影响

Table 1 Effects of the concentration of different enzymes on protoplast isolation from young leaves of tea plant

处理 Treatment 酶解液浓度 Concentration of enzyme (%) 产量 Yield (×106 g-1 FW) 活力 Viability (%) 纤维素酶 Cellulase 离析酶 Macerozyme 果胶酶 Pectolase 1 1.4 0.1 0.5 6.8 70 2 1.5 0.1 0.5 8.8 88 3 1.6 0.1 0.5 7.2 75 4 1.5 0 0.5 2.4 50 5 1.5 0.3 0.5 7.6 80 6 1.5 0.1 0.1 3.6 60 7 1.5 0.1 0.3 8.0 85 8 1.5 0.1 0.7 6.0 65