茶树叶片和胚根原生质体的分离及PEG诱导融合

彭章, 童华荣, 梁国鲁, 石艺琦, 袁连玉

Protoplast Isolation and Fusion Induced by PEG with Leaves and Roots of Tea Plant (Camellia sinensis L. O. Kuntze)

Zhang PENG, Hua-Rong TONG, Guo-Lu LIANG, Yi-Qi SHI, Lian-Yu YUAN

表2 不同酶解液组合对分离茶树胚根原生质体的影响

Table 2 Effects of the concentration of different enzymes on protoplast isolation from radicle of tea plant

处理 Treatment 酶解液浓度 Concentration of enzyme (%) 产量 Yield (×106 g-1 FW) 活力 Viability (%) 纤维素酶 Cellulase 离析酶 Macerozyme 果胶酶 Pectolase 1 1.4 0.3 0.5 2.8 83 2 1.5 0.3 0.5 3.2 89 3 1.6 0.3 0.5 3.0 85 4 1.5 0 0.5 1.0 50 5 1.5 0.1 0.5 2.4 75 6 1.5 0.3 0.3 2.0 70 7 1.5 0.3 0.7 2.4 76