茶树叶片和胚根原生质体的分离及PEG诱导融合

彭章, 童华荣, 梁国鲁, 石艺琦, 袁连玉

Protoplast Isolation and Fusion Induced by PEG with Leaves and Roots of Tea Plant (Camellia sinensis L. O. Kuntze)

Zhang PENG, Hua-Rong TONG, Guo-Lu LIANG, Yi-Qi SHI, Lian-Yu YUAN

表3 不同酶解时间对茶树叶片原生质体的产量和活力的影响

Table 3 Effects of enzymolysis time on protoplast yield and viability of tea leaves

酶解时间 Enzymolysis time (h) 原生质体数 Protoplast count (×106 g-1 FW) 活力 Viability (%) 6 6.8 80 7 8.8 88 8 8.0 75 9 6.0 50