中国小麦品种抗赤霉病基因Fhb1的鉴定与溯源

朱展望, 徐登安, 程顺和, 高春保, 夏先春, 郝元峰, 何中虎

Characterization of Fusarium Head Blight Resistance Gene Fhb1 and Its Putative Ancestor in Chinese Wheat Germplasm

Zhan-Wang ZHU, Deng-An XU, Shun-He CHENG, Chun-Bao GAO, Xian-Chun XIA, Yuan-Feng HAO, Zhong-Hu HE

表1 用于扩增PFT、HC和His基因开放阅读框的特异引物

Table 1 Specific primers used to amplify the open reading frames (ORF) of PFT, His, and HC

引物 Primer 序列 Sequence (5′-3′) 退火温度 Tm (°C) 目的片段 Target fragment PFT-1 F: ACAGGCACACACGGCTATAAATACC 61 PFT ORF片段1 Part 1 of PFT ORF R: AGATGGCGACTCTGCTAGACTATCA PFT-2 F: GCAACTCTATCAACATCGTCAATCTACC 61 PFT ORF片段2 Part 2 of PFT ORF R: AGAACTGGATAGCACGCAAGCATAT PFT-3 F: AGGCGGCATTATTGATGTTGAGGTAA 61 PFT ORF片段3 Part 3 of PFT ORF R: GGTTTCACCTCTCACGACCCATG HC3B-3 F: CAACCCAGTATCCGATACTTGTATAA 63 HC ORF R: CTGCTCCAAGCAAGCACGTA His3B-4 F: ATGCGTGCGCTGTACTTG 65 His ORF R: CGTCACAGAGTCCAGTGAAA