amiRNA技术沉默C-3氧化酶编码基因StCPD对马铃薯抗旱性的影响

周香艳, 杨江伟, 唐勋, 文义凯, 张宁, 司怀军

Effect of Silencing C-3 Oxidase Encoded Gene StCPD on Potato Drought Resistance by amiRNA Technology

Xiang-Yan ZHOU, Jiang-Wei YANG, Xun TANG, Yi-Kai WEN, Ning ZHANG, Huai-Jun SI

表3 马铃薯qRT-PCR分析相关基因引物

Table 3 Gene-specific primers sequences for qRT-PCR analysis of potato

引物Primer 引物序列Primer sequence (5'-3') amiRcpd 正向引物 amiRcpd forward primer TACGAATCGCTATCATAAT 18S RNA TTAGAGGAAGGAGAAGTCGTAACAA 通用反向引物 Universal reverse primer miRNA cDNA synthesis试剂盒提供 miRNA cDNA synthesis kit provided