甘蔗品种的AFLP和SSR标记鉴定及其应用

汪洲涛, 游倩, 高世武, 王春风, 李竹, 马晶晶, 阙友雄, 许莉萍, 罗俊

Identification of Sugarcane Varieties by AFLP and SSR Markers and Its Application

Zhou-Tao WANG, Qian YOU, Shi-Wu GAO, Chun-Feng WANG, Zhu LI, Jing-Jing MA, You-Xiong QUE, Li-Ping XU, Jun LUO

表2 15对SSR标记引物

Table 2 Fifteen pairs of primers used in SSR mark

引物名 Primer name 重复单元 Repeating unit 引物序列(正/反) Primer sequence (Positive/Reverse) (5°-3°) 退火温度 Tm (°C) SMC334BS (TG)36 CAATTCTGACCGTGCAAAGAT/CGATGAGCTTGATTGCGAATG 60 SMC336BS (TG)23(AG)19 ATTCTAGTGCCAATCCATCTCA/CATGCCAACTTCCAAACAGAC 62 SMC286CS (TG)43 TCAAATGGGACCTTATTGGAG/TCCCTCGATCTCCGTTGTT 58 SMC569CS (TG)37 GCGATGGTTCCTATGCAACTT/TTCGTGGCTGAGATTCACACTA 62 SMC119CG (TTG)12 TTCATCTCTAGCCTACCCCAA/AGCAGCCATTTACCCAGGA 58 SMC31CUQ (TC)10(AC)22 CATGCCAACTTCCAATACAGACT/AGTGCCAATCCATCTCAGAGA 62 mSSCIR43 (GT)3(AT)2(GT)29 ATTCAACGATTTTCACGAG/AACCTAGCAATTTACAAGAG 52 SEP17 (CCT)9 ACCCTGCTGGTCTCCTCC/ACGTTCGACGTCGTGTAGTG 60 SEP23 (AGA)9 GTGTTCAGGCAGATGGTCCT/GTCGATGGCACCGATTTATT 60 SEP59 (CGG)9 TTCCATTTACTCCTCCGTGC/CTCCCCCTCCTCGTACTTGT 60 SEP6 (GCA)5(ACA)13 CAGCCCATTAACCAAGCAAT/GAAGCAGCTGTTGCTCACTG 60 SEP70 (GCG)8 AACTCACCCAACAAAGCGAC/AGACGAAGAGCTCGTGGATG 60 SEP8 (CT)17 CTTGCTTCCCCTTTACTCCC/GAGGCGCCTTACTGTTCTTG 60 SEP84 (GGT)9 AGAGACCGTAATGGTGACCG/ACCACCACCACCACCATACT 60 SEP89 (CGT)10 AGCTCTGATTTTTGGGGGTT/GGAAGACAGTGGACGAGGTC 60