甘蔗品种的AFLP和SSR标记鉴定及其应用

汪洲涛, 游倩, 高世武, 王春风, 李竹, 马晶晶, 阙友雄, 许莉萍, 罗俊

Identification of Sugarcane Varieties by AFLP and SSR Markers and Its Application

Zhou-Tao WANG, Qian YOU, Shi-Wu GAO, Chun-Feng WANG, Zhu LI, Jing-Jing MA, You-Xiong QUE, Li-Ping XU, Jun LUO

表3 24对引物的高效性评价

Table 3 Effectiveness evaluation of 24 pairs of primers

标记类别 Marker sort 引物名 Primer name 多态性位点数 Number of polymorphic loci 总扩增位点数 Total site number 多态比例 Polymorphic percentage (%) 引物多态信息含量 PIC SSR标记 SSR marker (100-350 bp) SEP6 9 9 100 0.964 SPE8 13 14 92.86 0.959 SEP17 10 10 100 0.961 SEP23 22 22 100 0.979 SEP59 10 11 90.91 0.920 SEP70 8 8 100 0.895 SEP84 5 5 100 0.695 SEP89 12 12 100 0.896 SMC334BS 10 12 83.33 0.927 SMC336BS 12 12 100 0.967 SMC286CS 20 20 100 0.961 SMC569CS 9 9 100 0.779 SMC119CG 13 13 100 0.961 SMC31CUQ 11 11 100 0.967 mSSCIR43 12 12 100 0.973 总数/平均数Total/average 176 180 97.78 标记类别 Marker sort 引物名 Primer name 多态性位点数 Number of polymorphic loci 总扩增位点数 Total site number 多态比例 Polymorphic percentage (%) 引物多态信息含量 PIC AFLP标记 AFLP marker (1-500 bp) E-ACA/M-CTG 37 58 63.79 0.974 E-ACT/M-CAA 24 42 57.14 0.968 E-AAC/M-CAC 26 36 72.22 0.968 E-ACT/M-CAG 24 32 75.00 0.971 E-ACA/M-CAG 22 27 81.48 0.968 E-ACT/M-CAC 19 25 76.00 0.972 E-AAC/M-CAC 29 37 78.38 0.972 E-ACT/M-CTC 36 51 70.59 0.974 E-ACC/M-CAG 31 40 77.50 0.974 总数/平均数Total/average 248 348 71.26 0.971