小麦近缘属植物1-FFT基因的克隆及功能分析 |
王建伟,贺晓岚,李文旭,陈新宏 |
Molecular Cloning and Functional Analysis of 1-FFT in Wheat Relatives |
Jian-Wei WANG,Xiao-Lan HE,Wen-Xu LI,Xin-Hong CHEN |
图2 华山新麦草、簇毛麦和大赖草1-FFT基因PCR扩增产物电泳分析 M1: DL2000; M2: marker Ш. a: 基因保守区段扩增产物; b: 3¢ RACE扩增产物; c: 5¢ RACE 扩增产物; d: Ph-1-FFT (1~3泳道)、Dv-1-FFT (4~6泳道)和Lr-1-FFT (7~9泳道)的cDNA。 |
Fig. 2 Electrophoresi s analysis of PCR products of 1-FFT gene cloned from P. huashanica, D. villosum, and L. racemosus M1: DL2000; M2: marker Ш. a: amplification of gene conserved fragment; b: PCR product of 3¢ RACE; c: PCR product of 5¢ RACE; d: cDNA of Ph-1-FFT (lanes 1-3), Dv-1-FFT (lanes 4-6), and Lr-1-FFT (lanes 7-9). |
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