小麦近缘属植物1-FFT基因的克隆及功能分析

王建伟,贺晓岚,李文旭,陈新宏

Molecular Cloning and Functional Analysis of 1-FFT in Wheat Relatives

Jian-Wei WANG,Xiao-Lan HE,Wen-Xu LI,Xin-Hong CHEN

图2 华山新麦草、簇毛麦和大赖草1-FFT基因PCR扩增产物电泳分析 M1: DL2000; M2: marker Ш. a: 基因保守区段扩增产物; b: 3￠ RACE扩增产物; c: 5￠ RACE 扩增产物; d: Ph-1-FFT (1~3泳道)、Dv-1-FFT (4~6泳道)和Lr-1-FFT (7~9泳道)的cDNA。

Fig. 2 Electrophoresi s analysis of PCR products of 1-FFT gene cloned from P. huashanica, D. villosum, and L. racemosus M1: DL2000; M2: marker Ш. a: amplification of gene conserved fragment; b: PCR product of 3￠ RACE; c: PCR product of 5￠ RACE; d: cDNA of Ph-1-FFT (lanes 1-3), Dv-1-FFT (lanes 4-6), and Lr-1-FFT (lanes 7-9).