甘蔗磷脂酰肌醇转运蛋白基因ScSEC14响应干旱和盐胁迫

毛花英,刘峰,苏炜华,黄宁,凌辉,张旭,王文举,李聪娜,汤翰臣,苏亚春,阙友雄

A Sugarcane Phosphatidylinositol Transfer Protein Gene ScSEC14 Responds to Drought and Salt Stresses

Hua-Ying MAO,Feng LIU,Wei-Hua SU,Ning HUANG,Hui LING,Xu ZHANG,Wen-Ju WANG,Cong-Na LI,Han-Chen TANG,Ya-Chun SU,You-Xiong QUE

表2 ScSEC14基因克隆与表达所用引物

Table 2 Primers used in ScSEC14 gene cloning and expression analysis

引物 Primer 引物序列 Sequence information (5'-3') 用途 Purpose SEC14 F: AGGAAGCGCACAAGAACAGA 基因克隆 Gene cloning R: GGGAGTACAAGTCTCCTTGCATA qSEC14 F: CCACGAGTCACTTCCACACT 荧光定量Real-time-qPCR R: TGGGACCAAGAGAGTCCTGA CUL F: TGCTGAATGTGTTGAGCAGC 内参基因 Reference genes R: TTGTCGCGCTCCAAGTAGTC CAC F: ACAACGTCAGGCAAAGCAAA 内参基因 Reference genes R: AGATCAACTCCACCTCTGCG G-SEC14 F: GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCGGCCACCTCCGGAAGG 载体构建 Vector construction R: GGGGACCACTTTGTACAAGAAAGCTGGGTCTGGACCTTCGATCTGTATGCTG