基于CRISPR/Cas9技术的水稻温敏不育基因tms5突变体的构建

黄忠明,周延彪,唐晓丹,赵新辉,周在为,符星学,王凯,史江伟,李艳锋,符辰建,杨远柱

Construction of tms5 Mutants in Rice Based on CRISPR/Cas9 Technology

Zhong-Ming HUANG,Yan-Biao ZHOU,Xiao-Dan TANG,Xin-Hui ZHAO,Zai-Wei ZHOU,Xing-Xue FU,Kai WANG,Jiang-Wei SHI,Yan-Feng LI,Chen-Jian FU,Yuan-Zhu YANG

表1 本研究所用的引物

Table 1 Primer sequences used in this study

引物名称 Primer name 引物序列 Primer sequence (5°-3°) Target-1F TGGCGAACAGCGGCAAGTCATCGC Target -1R AAACGCGATGACTTGCCGCTGTTC M13F GTAAAACGACGGCCAG GUS-JC-F CGTCCGTCCTGTAGAAACCC GUS-JC-R GTGCGGATTCACCACTTGC TMS5-CX-F TCCAACGCATAGCAGTAGTCG TMS5-CX-R TGCCATCGTATCTCCGGTAAA