基于CRISPR/Cas9技术的水稻温敏不育基因tms5突变体的构建

黄忠明,周延彪,唐晓丹,赵新辉,周在为,符星学,王凯,史江伟,李艳锋,符辰建,杨远柱

Construction of tms5 Mutants in Rice Based on CRISPR/Cas9 Technology

Zhong-Ming HUANG,Yan-Biao ZHOU,Xiao-Dan TANG,Xin-Hui ZHAO,Zai-Wei ZHOU,Xing-Xue FU,Kai WANG,Jiang-Wei SHI,Yan-Feng LI,Chen-Jian FU,Yuan-Zhu YANG

表2 野生型(WT)和tms5突变体的农艺性状

Table 2 Agronomic traits of the wild-type (WT) and tms5 mutant grown in paddy ?eld

株系 Line 株高 Plant height (cm) 千粒重 1000-grain weight (g) 有效穗 Panicle number per plant 结实率 Seed setting rate (%) 单株产量 Grain yield per plant (g) WT 78.96±3.56 20.82±0.97 9.3±1.87 72.21±3.57 17.30±1.09 tms5 78.57±3.87 21.20±0.88 9.85±1.79 61.60±3.43** 15.22±1.05**