基于CRISPR/Cas9技术的水稻温敏不育基因tms5突变体的构建

黄忠明,周延彪,唐晓丹,赵新辉,周在为,符星学,王凯,史江伟,李艳锋,符辰建,杨远柱

Construction of tms5 Mutants in Rice Based on CRISPR/Cas9 Technology

Zhong-Ming HUANG,Yan-Biao ZHOU,Xiao-Dan TANG,Xin-Hui ZHAO,Zai-Wei ZHOU,Xing-Xue FU,Kai WANG,Jiang-Wei SHI,Yan-Feng LI,Chen-Jian FU,Yuan-Zhu YANG

图2 CRISPR/Cas9-TMS5载体构建示意图 A: 18T-Cas9-TMS5-gRNA重组载体结构示意图; B: pCAMBIA1301载体的T-DNA片段; C: pCAMBIA1301-Cas9-TMS5-gRNA重组载体结构示意图。LB: 左边界; RB: 右边界。

Fig. 2 Schematic diagram of the CRISPR/Cas9-TMS5 vector construction A: schematic diagram of the recombinant vector of 18T-Cas9-TMS5-gRNA; B: T-DNA fragment of pCAMBIA1301 vector; C: schematic diagram of the pCAMBIA1301-Cas9-TMS5-gRNA construct. LB: left border; RB: right border.