基于CRISPR/Cas9技术的水稻温敏不育基因tms5突变体的构建

黄忠明,周延彪,唐晓丹,赵新辉,周在为,符星学,王凯,史江伟,李艳锋,符辰建,杨远柱

Construction of tms5 Mutants in Rice Based on CRISPR/Cas9 Technology

Zhong-Ming HUANG,Yan-Biao ZHOU,Xiao-Dan TANG,Xin-Hui ZHAO,Zai-Wei ZHOU,Xing-Xue FU,Kai WANG,Jiang-Wei SHI,Yan-Feng LI,Chen-Jian FU,Yuan-Zhu YANG

图4 转基因植株的鉴定 A: PCR检测阳性转基因植株; B: GUS组织化学染色检测阳性转基因植株。M: DL2000; +: 阳性对照; 1-18: 转基因植株。

Fig. 4 Identification of the transgenic plants A: identification of the positive transgenic plants by PCR; B: identification of the positive transgenic plants by GUS histochemical staining. M: DL2000; +: positive control; 1-18: transgenic plants.