基于CRISPR/Cas9技术的水稻温敏不育基因tms5突变体的构建

黄忠明,周延彪,唐晓丹,赵新辉,周在为,符星学,王凯,史江伟,李艳锋,符辰建,杨远柱

Construction of tms5 Mutants in Rice Based on CRISPR/Cas9 Technology

Zhong-Ming HUANG,Yan-Biao ZHOU,Xiao-Dan TANG,Xin-Hui ZHAO,Zai-Wei ZHOU,Xing-Xue FU,Kai WANG,Jiang-Wei SHI,Yan-Feng LI,Chen-Jian FU,Yuan-Zhu YANG

图5 T0代转基因植株TMS5纯合突变类型和频率 A: 纯合突变类型序列比对; B: 纯合突变类型不同碱基插入的频率。红色字母为靶点序列; 蓝色字母为PAM序列; 绿色的字母为插入的碱基。

Fig. 5 Mutation types and frequencies at the TMS5 loci in T0 transgenic plants A: Sequence alignment of homozygous mutation types; B: Frequency of different base inserts in homozygous mutation types. The red letters are the target genome sequences; The blue letters are PAM; The green letters are the insert base.