基于CRISPR/Cas9技术的水稻温敏不育基因tms5突变体的构建

黄忠明,周延彪,唐晓丹,赵新辉,周在为,符星学,王凯,史江伟,李艳锋,符辰建,杨远柱

Construction of tms5 Mutants in Rice Based on CRISPR/Cas9 Technology

Zhong-Ming HUANG,Yan-Biao ZHOU,Xiao-Dan TANG,Xin-Hui ZHAO,Zai-Wei ZHOU,Xing-Xue FU,Kai WANG,Jiang-Wei SHI,Yan-Feng LI,Chen-Jian FU,Yuan-Zhu YANG

附图1 靶位点突变序列分析 红色的字母为靶点序列; 蓝色的字母为PAM序列; 绿色的字母为插入的碱基; +表示插入; WT表示野生型; Ho表示纯合突变; He表示杂合突变。

Supplementary Fig. 1 Analysis of mutation sequences of target The red letters are the target genome sequences; the blue letters are PAM; the green letters are the insert base; +: Insertion; WT: Wild-type; Ho: homozygous mutation; He: heterozygous mutation.