马铃薯块茎花色素苷合成相关R2R3 MYB蛋白基因的克隆和功能 分析

谈欢,刘玉汇,李丽霞,王丽,李元铭,张俊莲

Cloning and Functional Analysis of R2R3 MYB Genes Involved in Anthocyanin Biosynthesis in Potato Tuber

Huan TAN,Yu-Hui LIU,Li-Xia LI,Li WANG,Yuan-Ming LI,Jun-Lian ZHANG

表1 定量PCR分析的引物序列

Table 1 Primer sequences for qRT-PCR in this study

基因 Gene 登录号 Accession number 上游引物 Forward primer (5'-3') 下游引物 Reverse primer (5'-3') StAN1 AY841129.1 GGCCACATATCAAGAGAGGTGACTTTG TCACATCGTTAGCTGTCCTTCCTGG NtCHS AF311783.1 TTGTTCGAGCTTGTCTCTGC AGCCCAGGAACATCTTTGAG NtCHI AB213651.1 GTCAGGCCATTGAAAAGCTC CTAATCGTCAATGCCCCAAC NtF3H AB289450.1 CAAGGCATGTGTGGATATGG TGTGTCGTTTCAGTCCAAGG NtF3'H AB289449.1 AGGCTCAACACTTCTCGT CATCAACTTTGGGCTTCT NtDFR EF421429.1 AACCAACAGTCAGGGGAATG TTGGACATCGACAGTTCCAG NtANS AB289447.1 TGGCGTTGAAGCTCATACTG GGAATTAGGCACACACTTTGC NtUFGT FG627024.1 GAGTGCATTGGATGCCTTTT CCAGCTCCATTAGGTCCTTG NtbHLH HQ589208.1/HQ589209.1 ATGKGCGCAAACGAGGTTGATAGC TRGCTGAGGTTGTTGTTGCTCA NtEF-1α D63396.1 TGAACCATCCAKGACAGATTGG TGGGCTCCTTCTCAATCTCCTT