抗病转基因水稻M12及其产品成分的定性、定量PCR检测方法

李鹏,张琳,叶吉妮,贺诗瑶,贾军伟,潘爱虎,唐雪明

A Qualitative and Quantitative PCR Detection Method for Disease-resistant Genetically Modified Rice M12 and Its Derivates

Peng LI,Lin ZHANG,Ji-Ni YE,Shi-Yao HE,Jun-Wei JIA,Ai-Hu PAN,Xue-Ming TANG

表1 定性、定量PCR反应所用引物

Table 1 Primers used in qualitative (quantitative) PCR systems

引物 Primer 引物序列 Primer sequence (5°-3°) sps-F TTGCGCCTGAACGGATAT [13] sps-R CGGTTGATCTTTTCGGGATG [13] M12-SF GTTGGAGATTTTGGGCTTG [2] M12-SR ATAGCCTCTCCACCCAAGCG [2] M12-F GTTGGAGATTTTGGGCTTGC M12-R CGGCAAGAAACCATCCAGTT