抗病转基因水稻M12及其产品成分的定性、定量PCR检测方法

李鹏,张琳,叶吉妮,贺诗瑶,贾军伟,潘爱虎,唐雪明

A Qualitative and Quantitative PCR Detection Method for Disease-resistant Genetically Modified Rice M12 and Its Derivates

Peng LI,Lin ZHANG,Ji-Ni YE,Shi-Yao HE,Jun-Wei JIA,Ai-Hu PAN,Xue-Ming TANG

表2 M12定量PCR检测方法的检测极限和定量极限分析

Table 2 Limits of detection and quantification (LOD and LOQ) of M12 gene quantitative PCR

拷贝数 Copy 出现次数/检测次数 Signal rate (positive signals) 循环数平均值 Ct mean 标准差 SD 10000000 9/9 6.54 0.256 1000000 9/9 9.72 0.324 100000 9/9 13.15 0.236 10000 9/9 17.53 0.103 1000 9/9 20.82 0.265 100 9/9 24.12 0.231 10 2/9