抗病转基因水稻M12及其产品成分的定性、定量PCR检测方法

李鹏,张琳,叶吉妮,贺诗瑶,贾军伟,潘爱虎,唐雪明

A Qualitative and Quantitative PCR Detection Method for Disease-resistant Genetically Modified Rice M12 and Its Derivates

Peng LI,Lin ZHANG,Ji-Ni YE,Shi-Yao HE,Jun-Wei JIA,Ai-Hu PAN,Xue-Ming TANG

表3 M12和sps基因定量PCR检测体系的重复性分析

Table 3 Repeatability of M12 and sps gene quantitative PCR assays

质粒分子DNA拷贝数 Copies of plasmid DNA sps M12 循环数平均值 Ct mean 循环数标准差 Ct SD 循环数平均值 Ct mean 循环数标准差 Ct SD 1000000 12.15 0.087 6.52 0.276 100000 15.30 0.076 9.72 0.154 10000 18.62 0.067 13.13 0.186 10000 21.91 0.043 17.52 0.133 1000 24.32 0.123 20.71 0.165 100 27.60 0.124 24.05 0.134