抗病转基因水稻M12及其产品成分的定性、定量PCR检测方法

李鹏,张琳,叶吉妮,贺诗瑶,贾军伟,潘爱虎,唐雪明

A Qualitative and Quantitative PCR Detection Method for Disease-resistant Genetically Modified Rice M12 and Its Derivates

Peng LI,Lin ZHANG,Ji-Ni YE,Shi-Yao HE,Jun-Wei JIA,Ai-Hu PAN,Xue-Ming TANG

图3 M12事件特异性定性PCR的特异性检测 M12品系特异性扩增(A)和水稻内标准基因sps扩增(B)。M: DNA marker (DL1000); 1: M12质粒pM12; 2: M12; 3: 花优14; 4: 华恢1号; 5: 旱优3号; 6: 旱恢3T; 7: 阴性对照。

Fig. 3 Event-specific detection of qualitative PCR for M12: The qualitative PCR of event-specific M12 (A) and rice endogenous gene sps (B). M: DNA marker (DL1000); 1: pM12; 2: rice variety M12; 3: rice variety Huayou 14; 4: rice variety Huahui 1; 5: rice variety Hanyou 3; 6: rice variety Hanhui 3T; 7: negative control.