高分子量麦谷蛋白亚基HPCE高效分离及图谱鉴定

王卫东,高翔,赵丹阳

Efficient Separation and Identification of High Molecular Weight Glutenin Subunits by HPCE

Wei-Dong WANG,Xiang GAO,Dan-Yang ZHAO

表1 HMW-GS分子标记

Table 1 Molecular markers of HMW-GS

亚基 Subunit 标记序列 Sequence (5′→3′) 片段大小 Size of fragment (bp) 参考文献 Reference 1Ax1 F: TCACCGACAGTCCACCGA; R: ACCAAGCGAGCTGCAGAG 2532 Bustos et al. [16] Null F: ACGTTCCCCTACAGGTACTA; R: TATCACTGGCTAGCCGACAA 920 Lafiandra et al. [17] 1Ax2* F: ATGACTAAGCGGTTGGTTCTT; R: ACCTTGCTCCCCTTGTCTTT 1319 Ma et al. [18] 1Bx6 F: CACTGAGATGGCTAAGCGCC; R: GCCTTGGACGGCACCACAGG 321 Schwarz et al. [19] 1Bx7/1Bx17 F: CGCAACAGCGAGGACAATT; R: TGGTCCGTCACTATCTTGAGA 766/669 Ma et al. [18] 1Bx13 F: ATGAGCTAAGCGCGCTGGTCCTCTTTG; R: CTATCACTGCCTGGTCCGACAATGCG 900 Pang and Zhang [20] 1Bx20 F: CCTCAGCATGCAAACATGCAGC; R: CTGAAACCTTTGGCCAGTCATGTC 800 Butow et al. [21] 1By8 F: TTAGCGCTAAGTGCCGTCT; R: TTGTCCTATTTGCTGCCCTT 527 Lei et al. [22] 1By9 F: TTCTCTGCATCAGTCAGGA; R: AGAGAAGCTGTGTAATGCC 707 Lei et al. [22] 1By16 F: GCAGTACCCAGCTTCTCAA; R: CCTTGTCTTGTTTGTTGCC 3 fragments Lei et al. [22] 1By18 F: CAACAAAACGGGCGTTGT; R: CAACAAAACGGGCGTTGT 365 Liang et al. [23] 1Dx2/1Dx5 F: GGGACAATACGAGCAGCAAA; R: CTTGTTCCGGTTGTTGCCA 299/281 Liu et al. [24] 1Dy10/1Dy12 F: GTTGGCCGGTCGGCTGCCATG; R: TGGAGAAGTTGGATAGTACC 576/612 Ahmad [25]