高分子量麦谷蛋白亚基HPCE高效分离及图谱鉴定

王卫东,高翔,赵丹阳

Efficient Separation and Identification of High Molecular Weight Glutenin Subunits by HPCE

Wei-Dong WANG,Xiang GAO,Dan-Yang ZHAO

图2 缓冲液组分浓度、pH对HMW-GS电泳分离的影响 A: 不同pH对分离效果的影响, 缓冲液组分为100 mmol L-1 IDA+0.05% HPMC+20% ACN。B: 缓冲液不同IDA浓度下连续两针电泳分离结果, 其他组分为0.05% HPMC+20% ACN, pH 2.5。C: 缓冲液不同HPMC浓度对分离效果的影响, 其他组分为75 mmol L-1 IDA+20% ACN, pH 2.5。D: 缓冲液不同ACN浓度对分离效果的影响, 其他组分为75 mmol L-1 IDA+0.05% HPMC, pH 2.5。A~D电泳参数均为毛细管内径50 μm, PDA检测波长214 nm, 分离电压15 kV, 运行温度25 °C。箭头所示为副峰。

Fig. 2 Effect of constituent concentration and pH values of buffer on electrophoresis separation of HMW-GS A: effect of different pH on the electrophoresis separation, the constituents of the buffer were 100 mmol L-1 IDA + 0.05% HPMC + 20% ACN. B: results of two successive electrophoretic separation under different concentrations of IDA, the other constituents of the buffer were 0.05% HPMC + 20% ACN, the pH was 2.5. C: effect of different concentrations of HPMC on the electrophoresis separation, the other constituents of the buffer were 75 mmol L-1 IDA + 20% ACN, the pH was 2.5. D: effect of different concentrations of ACN on the electrophoresis separation, the other constituents of the buffer were 75 mmol L-1 IDA + 0.05% HPMC, the pH was 2.5. Electrophoresis parameters of A-D: the inner diameter of the capillary was 50 μm; the detection wavelength was 214 nm; the separation voltage was 15 kV; the operating temperature was 25 °C. The arrow indicates the position of the secondary peak.