高分子量麦谷蛋白亚基HPCE高效分离及图谱鉴定

王卫东,高翔,赵丹阳

Efficient Separation and Identification of High Molecular Weight Glutenin Subunits by HPCE

Wei-Dong WANG,Xiang GAO,Dan-Yang ZHAO

图3 电泳参数优化前(A)和优化后(B)的HPCE图谱 缓冲液成分: 75 mmol L-1 IDA + 0.05% HPMC + 15% ACN, pH 2.5。优化前电泳参数: 毛细管内径50 μm, PDA检测波长214 nm, 分离电压15 kV, 运行温度25°C; 优化后电泳参数: 毛细管内径25 μm, PDA检测波长200 nm, 分离电压20 kV, 运行温度30°C。

Fig. 3 HPCE profile before (A) and after (B) optimization of electrophoresis parameters Buffer constituents: 75 mmol L-1 IDA + 0.05% HPMC + 15% ACN, pH 2.5. Electrophoresis parameters were 50 μm of the inner diameter of capillary, 214 nm of detection wavelength, 15 kV of separation voltage, and 25°C of operating temperature before optimization and 25 μm of the inner diameter of capillary, 200 nm of the detection wavelength, 20 kV of separation voltage, and 30°C of operating temperature after optimization.