水稻抗稻瘟病基因Pi47的精细定位和候选基因分析
肖湘谊,史学涛,盛浩闻,刘金灵,肖应辉

Fine Mapping and Candidate Gene Analysis of Rice Blast Resistance Gene Pi47
Xiang-Yi XIAO,Xue-Tao SHI,Hao-Wen SHENG,Jin-Ling LIU,Ying-Hui XIAO
表4 Pi47精细定位使用的标记信息
Table 4 Molecular markers used for Pi47 fine mapping
标记
Marker
引物顺序
Primer sequence (5°-3°)
物理位置
Physical position
扩增大小
Expect size

Enzyme
标记类型
Marker type
RM224 F: TCGATCGATCTTCACGAGG
R: TGCTATAAAAGGCATTCGGG
27673251
-27673372
122 / SSR
S11 F: TCTTTGAACAAGAGGACCAG
R: AATTACCGAATTACTTAGCG
27824419
-27825439
1021 Mva I or Hinf I CAPS
S4 F: CGAATGATGACGAGGACCCG
R: ATCAGGCACGGCAGCAGAAG
27827786
-27828843
1058 Hinf I or Rsa I CAPS
S32 F: GCCCAGTAACCGTAGGAGTC
R: TTTGATGAGCGGCAAAGTAG
27835604
-27836295
692 Msp I CAPS
S10 F: AGAGCAAGAAGGGCACGGTAT
R: AGAATGGTCCTCCTGACATCG
27992866
-27993970
1105 Rsa I CAPS
K33 F: TTTGGTGCTCCTCTTACGGG
R: TCGGAGTTCACAGAGCCAAG
28005690
-28006842
1153 Hinf I CAPS
K107 F: ACATCAATGGCTACAACT
R: TGCTAACGGTGCTGGTAT
28012753
-28012940
184/188 / STS
K134 F: GATGGCGAGATGGTTGTC
R: GCCTTTGAGATAGGGATTGC
28163563
-28163742
180/168 / STS
RM5926 F: TAGGTCCATCCAAATCTCGATCC
R: TGGCAGAGGAGATTAGAGTAATACGG
28957564
-28957858
295 / SSR
S6 F: TCAACCGAACGCAAGAATCAC
R: GGCCCTCCAGCTCATCTACCT
27841529
-27842431
903 / STS
SC1 F: CCCCACAATCCCACGAGAC
R: CGGGCCGACAATCCAATAC
27848711
-27849635
925 / STS
SC3 F: TTTCCCACCGATGACCCAG
R: GCTCGCCATCCTTCCCTTT
27879005
-27879906
902 / STS
SC6 F: TATTGGTAAGTGGTAACGGGTGA
R: GCAGATTCGAGGAGGAAGG
27891111
-27892550
1440 / STS
SC7 F: TGGCGTGGCGGTTTGGTAG
R: GCTGGTTGGGCATGGGTTG
27909896
-27910602
707 / STS
SC12 F: GGGCAACAAGCGAGCCATAA
R: GTGGTGAGGCAGCGGAACAG
27939030
-27940473
1444 / STS