大豆半胱氨酸蛋白酶抑制剂基因GmCYS2的功能鉴定
|
|
柯丹霞,彭昆鹏,贾妍,曾硕,王英枝,张静怡
|
Functional Characterization of Soybean Cystatins Gene GmCYS2
|
|
Dan-Xia KE,Kun-Peng PENG,Yan JIA,Shuo ZENG,Ying-Zhi WANG,Jing-Yi ZHANG
|
|
表1 本研究中所使用的引物
|
Table 1 Primers used in this study
|
|
引物名称
Primer name | 引物序列
Sequence of primer (5′-3′) | | F-GmCYS2-pro. | TACGGGGGATTGGTC | | R-GmCYS2-pro. | TCACTGCGTGGAAGGAG | | F-OX | CGggatccATGGCGGCGTTGATAAG | | R-OX | GGggtaccTCACTGCGTGGAAGGAG | | F-NIN-rt | AACTCACTGGAAACAGGTGCTTTC | | R-NIN-rt | CTATTGCGGAATGTATTAGCTAGA | | F-ENOD40-1-rt | GGAGGTATGCTCAAACATTC | | R-ENOD40-1-rt | GTAACTTCTCAAGAGAAGACC | | F-ENOD40-2-rt | CAAAACTCGTTATGTTGCGG | | R-ENOD40-2-rt | CACCTCAAAGGAAGAAGAACA | | F-GmCYS2-rt | CAACAAGTGGTGTCTG | | R-GmCYS2-rt | TCACTGCGTGGAAGGAG | | F-Polyubiquitin | TTCACCTTGTGCTCCGTCTTC | | R-Polyubiquitin | AACAACAGCACACACAGACAATC |
|
|
|