大豆半胱氨酸蛋白酶抑制剂基因GmCYS2的功能鉴定

柯丹霞,彭昆鹏,贾妍,曾硕,王英枝,张静怡

Functional Characterization of Soybean Cystatins Gene GmCYS2

Dan-Xia KE,Kun-Peng PENG,Yan JIA,Shuo ZENG,Ying-Zhi WANG,Jing-Yi ZHANG

图2 His-GST-GmCYS2融合蛋白的序列分析 氨基酸第5~10位: His标签; 第14~233位: GST标签; 第244~250位: TEV(烟草蚀纹病毒)切割位点; 251~355位: GmCYS2蛋白序列(去掉N端26个氨基酸编码的信号肽)。

Fig. 2 Sequence analysis of His-GST-GmCYS2 fusion protein 5-10: His tag; 14-233: GST tag; 244-250: TEV cleavage site; 251-355: GmCYS2 protein sequence (Removing signal peptide of 26 amino acids at N end).