大豆半胱氨酸蛋白酶抑制剂基因GmCYS2的功能鉴定

柯丹霞,彭昆鹏,贾妍,曾硕,王英枝,张静怡

Functional Characterization of Soybean Cystatins Gene GmCYS2

Dan-Xia KE,Kun-Peng PENG,Yan JIA,Shuo ZENG,Ying-Zhi WANG,Jing-Yi ZHANG

图4 重组蛋白GmCYS2在不同时期根瘤提取物中的抑制活性分析 样品分别为30 d和60 d根瘤提取物。反应液中加入纯化的GmCYS2蛋白, 另外分别加入组织蛋白酶L类、B类和H类3种不同底物, 试验重复3次。* P < 0.05。

Fig. 4 Inhibitory activity of the recombinant protein GmCYS2 in extracts from nodules in different periods in vitro Samples were extracts of 30-day- and 60-day-old nodules. Reaction buffer added with purified GmCYS2 protein and the specific substrates cathepsin L-like, B-like, and H-like, respectively. Values are triplicate measurements. * P < 0.05.