大豆半胱氨酸蛋白酶抑制剂基因GmCYS2的功能鉴定

柯丹霞,彭昆鹏,贾妍,曾硕,王英枝,张静怡

Functional Characterization of Soybean Cystatins Gene GmCYS2

Dan-Xia KE,Kun-Peng PENG,Yan JIA,Shuo ZENG,Ying-Zhi WANG,Jing-Yi ZHANG

图5 GmCYS2基因的过量表达对百脉根共生结瘤的影响 A: 复合体植株接种MAFF303099根瘤菌30 d后的结瘤表型。毛状根表达pMUb:GmCYS2 (GmCYS2-OX)或空载体pU1301 (CK), Bar = 10 mm; B: 单株复合体百脉根植株的平均结瘤数目; C: 根据单株根瘤数划分的不同结瘤种类所占的相对百分比; D: 阳性毛根的GUS鉴定; E: RT-PCR检测阳性毛根中GmCYS2基因的表达。M: DL2000 DNA marker; 1: 阳性质粒对照; 2: 空载体对照; 3~5: 复合体植株。

Fig. 5 Effect of GmCYS2 overexpression on symbiosis in L. japonicus A: Transgenic plants at 30 days after inoculation with MAFF303099 showing hairy roots expressing pMUb: GmCYS2 (GmCYS2-OX) or the empty vector pU1301 (CK). Bar = 10 mm; B: Mean numbers of nodules per plant of L. japonicus; C: The relative ratios of various groups divided by nodule number per plant; D: GUS identification of positive hairy roots; E: Detection of GmCYS2 gene expression in positive hairy roots by RT-PCR; M: DL2000 DNA marker; 1: plasmid as positive control; 2: empty vector control; 3-5: composite plants.