大豆引进种质抗胞囊线虫病、抗花叶病毒病和耐盐基因型鉴定及优异等位基因聚合种质筛选

叶俊华,杨启台,刘章雄,郭勇,李英慧,关荣霞,邱丽娟

Genotyping of SCN, SMV Resistance, Salinity Tolerance and Screening of Pyramiding Favorable Alleles in Introduced Soybean Accessions

Jun-Hua YE,Qi-Tai YANG,Zhang-Xiong LIU,Yong GUO,Ying-Hui LI,Rong-Xia GUAN,Li-Juan QIU

表1 抗SCN、抗SMV和耐盐相关标记的类型及引物序列

Table 1 Types of SCN resistance, SMV resistance, and salinity tolerance markers and primer sequences

性状 Trait 标记 Marker 标记类型 Type 基因 Gene 引物序列信息 Primer sequence (5°-3°) 等位基因 Allele 参考文献 Reference 大豆胞囊线虫抗性 SCN resistance Rhg4-389 KASP Glyma08g11490 Rhg4-COM: CTACACCGCCGTCCTCAAC [17] Rhg4-FAM: GAGGTGGCCGCCGGAGC G/G Rhg4-HEX: GAGGTGGCCGCCGGAGG C/C rhg1 KASP Glyma18g02590 rhg1-COM: CTGGCATCTGCCAACTCTGTAAAGA [18] rhg1-FAM: TTCTAATGCATTGGTTATAGCAACAACG C/C rhg1-HEX: TTCTAATGCATTGGTTATAGCAACAACC G/G SCN3-11 KASP Glyma11g35820 SCN3-11-COM: CAGAAGTCGATTGAGATTTACGAAGAGATA [9] SCN3-11-FAM: ATTATTGTTGAGGGATTGGCGAGC C/C SCN3-11-HEX: AAATTATTGTTGAGGGATTGGCGAGT T/T 大豆耐盐性 Soybean salinity tolerance SALT3 KASP Glyma03g32900 GmSALT3-COM: GATCATTGGATGTAATTGGGTGGAGAA [14] GmSALT3-FAM: GATACCAGCAAATATTAAATGTGTGTTTTT T/T GmSALT3-HEX: GATACCAGCAAATATTAAATGTGTGTTTTA -/- Ncl-5 KASP Glyma03g32900 Ncl-5-COM: AGGTACTTACCCTTATGAAGAAAACA [14] Ncl-5-FAM: AGAACTCGTATTTTATTTTGGTTGAC G/G Ncl-5-HEX: AGAACTCGTATTTTATTTTGGTTGAT A/A 大豆花叶病毒抗性 SMV resistance SCN11 SCAR — SCN11-F: TTCACGTGGCCCTCCTATC — [12] SCN11-R: CGCCGCAAACTCACAGGAC —