玉米隐花色素CRY1b和CRY2基因转录丰度对不同光质处理的响应

李红丹,闫蕾,孙蕾,樊晓聪,陈士瞻,张燕,郭林,游光霞,李庄,杨宗举,苏亮,杨建平

Transcription Abundances of CRY1b and CRY2 Genes in Response to Different Light Treatments in Maize

Hong-Dan LI,Lei YAN,Lei SUN,Xiao-Cong FAN,Shi-Zhan CHEN,Yan ZHANG,Lin GUO,Guang-Xia YOU,Zhuang LI,Zong-Ju YANG,Liang SU,Jian-Ping YANG

图4 ZmCRY1b和ZmCRY2基因在不同器官中的相对表达水平分析 分别取于自然条件下生长一定阶段的B73幼苗的不同器官(根、茎、叶、雄花、叶枕、叶鞘、花丝、幼穗、花柄和苞叶)为材料用于qRT-PCR分析。以根中的ZmCRY1b的转录丰度为对照, 将该ZmCRY1b/Tubulin的比值设为1。柱状图显示了在3次独立的生物学重复下ZmCRY1b/Tubulin比值的平均值, 其中, 误差线代表了标准差。用星号表示差异的显著性, * P < 0.05, ** P < 0.01。

Fig. 4 Relative expression analysis of both ZmCRY1b and ZmCRY2 genes in different organs of maize Different tissues of maize inbred line B73, including root, stem, leaf, stamen, pulvinus, sheath, pedicel, young ear, pistil and bract, were harvested for qRT-PCR assays. The transcription abundance of ZmCRY1b in the root was set as the control, and the ratio of ZmCRY1b/Tubulin in the root was set as 1. Each column shows the mean expression of ZmCRY1b/Tubulin of three biological repeats. Error bars indicate the standard deviation. * P < 0.05, ** P < 0.01.