玉米隐花色素CRY1b和CRY2基因转录丰度对不同光质处理的响应

李红丹,闫蕾,孙蕾,樊晓聪,陈士瞻,张燕,郭林,游光霞,李庄,杨宗举,苏亮,杨建平

Transcription Abundances of CRY1b and CRY2 Genes in Response to Different Light Treatments in Maize

Hong-Dan LI,Lei YAN,Lei SUN,Xiao-Cong FAN,Shi-Zhan CHEN,Yan ZHANG,Lin GUO,Guang-Xia YOU,Zhuang LI,Zong-Ju YANG,Liang SU,Jian-Ping YANG

图6 ZmCRY1b和ZmCRY2基因由黑暗到不同光质下的相对表达丰度 玉米自交系B73的幼苗在黑暗生长13 d后, 分别转入蓝光(B, 13 μmol m-2 s-1)、白光(W, 17 μmol m-2 s-1)、红光(R, 22.3 μmol m-2 s-1)下0 h、0.25 h、0.5 h、1 h、2 h、4 h、8 h、12 h和24 h以及远红光(FR, 0.25 μmol m-2 s-1)下0 h、0.16 h、0.25 h、0.5 h、1 h、2 h、4 h、8 h、12 h、24 h和24.08 h取材, 用于qRT-PCR分析。把黑暗条件下ZmCRY2/Tubulin的比值设为1, 并以此为对照。折线图代表了在3次独立的生物学重复下ZmCRY2/Tubulin的平均值, 其中误差线代表了标准差。

Fig. 6 Relative expression of both ZmCRY1b and ZmCRY2 genes during transitions from darkness to different light conditions The seedlings of maize inbred line B73 were grown in the dark for 13 d, then transferred to blue light (B, 13 μmol m-2 s-1), white light (W, 17 μmol m-2 s-1), or red light (R, 22.3 μmol m-2 s-1) for 0.25 h, 0.5 h, 1 h, 2 h, 4 h, 8 h, 12 h, or 24 h and far-red light (FR, 0.25 μmol m-2 s-1) for 0.16 h, 0.25 h, 0.5 h, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, or 24.08 h. The transcription abundance of ZmCRY2 in the dark was set as the control, and the ratio of ZmCRY2/Tubulin in the dark was set as 1. Each line graph shows the mean expression of ZmCRY2/Tubulin of three biological repeats. Error bars indicate the standard deviation.