大豆GmHDL57基因的克隆及抗盐功能鉴定

柯丹霞,彭昆鹏,张孟珂,贾妍,王净净

Cloning and Salt Resistance Function Identification of GmHDL57 Gene from Glycine max

Dan-Xia KE,Kun-Peng PENG,Meng-Ke ZHANG,Yan JIA,Jing-Jing WANG

图7 转基因百脉根阳性植株检测及抗盐表型鉴定 A: PCR检测植株中GUS基因的表达; B: RT-PCR检测植株中GmHDL57基因的表达; C: 不同盐浓度处理14 d后百脉根的生长状态。Lj 9-5, Lj 1-1: 转基因株系。M: DL2000 DNA marker。

Fig. 7 Positive plants detection and salt stress phenotype analysis of transgenic Lotus japonicus A: detection of GUS gene expression in plants by PCR; B: detection of GmHDL57 gene expression in plants by RT-PCR; C: growth state of Lotus japonicus in different salt concentration treatment for 14 d. Lj 9-5, Lj 1-1: transgenic lines. M: DL2000 DNA marker.