甘蔗ScWRKY4基因的克隆与表达特性分析

王玲,刘峰,戴明剑,孙婷婷,苏炜华,王春风,张旭,毛花英,苏亚春,阙友雄

Cloning and Expression Characteristic Analysis of ScWRKY4 Gene in Sugarcane

Ling WANG,Feng LIU,Ming-Jian DAI,Ting-Ting SUN,Wei-Hua SU,Chun-Feng WANG,Xu ZHANG,Hua-Ying MAO,Ya-Chun SU,You-Xiong QUE

表1 引物序列及用途

Table 1 Sequence and usage of primers

引物名称 Primer name 引物序列 Primer sequence (5°-3°) 用途 Usage ScWRKY4-F TCCTCGGCATCTCCCATTCT 扩增全长 Full length amplifcation ScWRKY4-R GTCTGGGAGCTCATGTTCGT ScWRKY4-QF ATGAAGGTGAGGAGGAAGATG 荧光定量 qRT-PCR ScWRKY4-QR CTTGTAGCCGTCGTCCAG GAPDH-QF CACGGCCACTGGAAGCA 内参基因 Reference gene GAPDH-QR TCCTCAGGGTTCCTGATGCC ScWRKY4-Subloc-F CGGGATCCATGGAGGGGAGCA 亚细胞定位 Subcellular localization ScWRKY4-Subloc-R TGCTCTAGAGAGCGACGTGAAAGC ScWRKY4-BD-F GGAATTCCATATGATGGAGGGGAGCAGCCAGCT 转录激活 Transactivation ScWRKY4-BD-R CGGGATCCGAGCGACGTGAAAGCGCAGC