甘蔗ScWRKY4基因的克隆与表达特性分析

王玲,刘峰,戴明剑,孙婷婷,苏炜华,王春风,张旭,毛花英,苏亚春,阙友雄

Cloning and Expression Characteristic Analysis of ScWRKY4 Gene in Sugarcane

Ling WANG,Feng LIU,Ming-Jian DAI,Ting-Ting SUN,Wei-Hua SU,Chun-Feng WANG,Xu ZHANG,Hua-Ying MAO,Ya-Chun SU,You-Xiong QUE

图10 甘蔗ScWRKY4基因在不同植物激素和非生物胁迫下的表达水平 柱上不同的小写字母代表显著性的差异(P ≤ 0.05); n = 3; 内参基因为甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphate dehydrogenase, GAPDH); SA: 水杨酸(5 mmol L-1); MeJA: 茉莉酸甲酯(25 μmol L-1); NaCl: 氯化钠(模拟盐胁迫) (250 mmol L-1); PEG: 聚乙二醇(模拟干旱胁迫) (25.0%); ABA: 脱落酸(100 μmol L-1)。

Fig. 10 Relative expression level of the sugarcane ScWRKY4 gene under different plant hormones and abiotic stress treatments Bars superscripted by different lowercase letters are significantly different (P ≤ 0.05); n = 3; Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a reference gene; SA: salicylic acid (5 mmol L-1); MeJA: methyl jasmonate (25 μmol L-1); NaCl: sodium chloride (simulating salt stress) (250 mmol L-1); PEG: polyethylene glycol (simulating drought treatment) (25.0%); ABA: abscisic acid (100 μmol L-1).