彩色棉多药和有毒化合物输出蛋白MATE家族基因的鉴定及表达分析

王作敏,刘瑾,孙士超,张新宇,薛飞,李艳军,孙杰

Identification and Expression Analysis of Multidrug and Toxic Compound Extrusion Protein Family Genes in Colored Cotton

Zuo-Min WANG,Jin LIU,Shi-Chao SUN,Xin-Yu ZHANG,Fei XUE,Yan-Jun LI,Jie SUN

表1 实验中所用的引物

Table 1 Primers used in the experiment

基因名称 Gene name 上游引物序列 Forward primer sequence (5′-3′) 下游引物序列 Reverse primer sequence (5′-3′) GhA11G0040 GCTGCCACGGTTTGGATGAT GCCTCTTGCTCCCAGTTTGT GhA08G2343 ATTGCTGGCTTGCTCCATACTT CCACTTGTTAACTCGCGATTGA GhA13G0797 TTTATCTCCGTTGTTGGCTTCT CCTGTACCGCGACAGTGAGC GhD05G1622 ATTGTATTTGCTCTGCGAGATGTTA TTGCACCGAGGTCGTACTTGA GhA12G0971 CATCCAAGGGCAGCCAAATA CCTCCTCCCACAGCAACACC GhA03G0955 GGCGGCAATAATAATAATGGT GGATAAGGGTTAATGCTAGAAGC GhA03G1186 GGTGTTATAGTATCCCTAATCCTCATC TTCTTTATCCTTTCCTCCGCAAT GhA05G1901 CGGTGCAGGAAATGCGAAAG AGCCCAGATACCAGTGATACCAAA GhA06G0259 GTTAGGCGTCACCATTCTACTCAA GTTCCTCCAAAGATCATCCCAC GhA10G0781 GGCAATCCTACGTGGCTTACAT TTTGCTTTCTCAGCCTCCTTCT GhD02G1620 TACGATAGCCCTTATTCTGATCATC TCTCTTTAGTGTCAACGTCACCTCC GhD11G2314 GAGGAAATGCAAAGGCAGTGAA CAATAACCATCGGTTGAGACGT GhD11G2334 AAGTAGCAAACTCCGTGGCAGAC TTCCCAATCAGTCCTTGAGGTAAT GhA03G1321 ATAGCAATGAGAAGGAAGTGGTGGAT TTGTAACAATGGCGAGCAGAAT GhD01G1006 TTACTTCCCTTACTTCTTCACTACCA CCCATTTCTTTATACGCCTTTC GhD13G2211 AACCGTGATAGGCACCGTCAT TCCAAAGTCCCTTCCCTCCA GhA09G0412 TGCTATGAGCGTTCGGGTTTC CATGCGTTCCGTTGTTTGTTC GhA04G0282 AATCGTTTCGTTTGGTCTTCGC ACACCTGCCATCCGCTTCCTCT GhA12G0812 UBQ7 AGCAGTTTCAGACTTGACTCCAC GAAGGCATTCCACCTGACCAAC GACTCCAATAATCATACCCCACC CTTGACCTTCTTCTTCTTGTGCTTG