彩色棉多药和有毒化合物输出蛋白MATE家族基因的鉴定及表达分析
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王作敏,刘瑾,孙士超,张新宇,薛飞,李艳军,孙杰
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Identification and Expression Analysis of Multidrug and Toxic Compound Extrusion Protein Family Genes in Colored Cotton
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Zuo-Min WANG,Jin LIU,Shi-Chao SUN,Xin-Yu ZHANG,Fei XUE,Yan-Jun LI,Jie SUN
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表2 陆地棉MATE蛋白家族成员的鉴定
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Table 2 Identification of MATE protein family members in Gossypium hirsutum L.
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基因 Gene | 序列号 Sequence number | 染色体 Chr. | 编码序列 Coding sequence (bp) | 蛋白残基数 Protein length (aa) | 分子量 Molecular weight (kD) | 等电点 pI | 亚细胞定位预测 Subcellular location prediction | GhMATE1 | GhA01G0453 | 1 | 1455 | 484 | 48.1 | 7.72 | 细胞膜Cell membrane | GhMATE2 | GhA01G0959 | 1 | 1455 | 484 | 49.3 | 8.62 | 细胞膜Cell membrane | GhMATE3 | GhA03G0955 | 3 | 1383 | 460 | 46.4 | 5.21 | 叶绿体Chloroplast | GhMATE4 | GhA03G1185 | 3 | 1554 | 517 | 48.1 | 9.05 | 细胞质Cytoplasm | GhMATE5 | GhA03G1186 | 3 | 1545 | 514 | 48.2 | 6.48 | 细胞膜Cell membrane | GhMATE6 | GhA03G1321 | 3 | 1470 | 489 | 47.1 | 7.58 | 液泡膜Tonoplast | GhMATE7 | GhA03G1323 | 3 | 1467 | 488 | 47.2 | 7.58 | 细胞膜Cell membrane | GhMATE8 | GhA04G0255 | 4 | 1275 | 424 | 45.3 | 8.62 | 细胞膜Cell membrane | GhMATE9 | GhA04G0282 | 4 | 1416 | 471 | 47.0 | 7.96 | 细胞膜Cell membrane | GhMATE10 | GhA05G1449 | 5 | 1575 | 524 | 48.0 | 5.85 | 细胞质Cytoplasm | GhMATE11 | GhA05G1901 | 5 | 1476 | 491 | 47.5 | 8.36 | 叶绿体Chloroplast | GhMATE12 | GhA06G0259 | 6 | 1479 | 492 | 48.5 | 8.36 | 细胞质Cytoplasm | GhMATE13 | GhA09G0073 | 9 | 1470 | 489 | 48.1 | 8.86 | 液泡膜Tonoplast | GhMATE14 | GhA08G2343 | 8 | 1497 | 498 | 53.7 | 5.60 | 细胞膜Cell membrane | GhMATE15 | GhA09G0412 | 9 | 1521 | 506 | 49.9 | 5.49 | 细胞膜Cell membrane | GhMATE16 | GhA10G0781 | 10 | 1458 | 485 | 49.3 | 5.55 | 细胞膜Cell membrane | GhMATE17 | GhA11G0039 | 11 | 1404 | 467 | 45.6 | 8.52 | 细胞膜Cell membrane | GhMATE18 | GhA11G0040 | 11 | 1533 | 510 | 48.5 | 8.65 | 细胞膜Cell membrane | GhMATE19 | GhA11G1853 | 11 | 1473 | 490 | 49.5 | 8.34 | 细胞膜Cell membrane | GhMATE20 | GhA11G2034 | 11 | 1452 | 483 | 48.7 | 8.58 | 细胞膜Cell membrane | GhMATE21 | GhA11G3233 | 11 | 1473 | 490 | 48.2 | 8.22 | 液泡膜Tonoplast | GhMATE22 | GhA11G3238 | 11 | 1419 | 472 | 48.8 | 8.43 | 细胞膜Cell membrane | GhMATE23 | GhA12G0812 | 12 | 1503 | 500 | 53.8 | 6.99 | 液泡膜Tonoplast | GhMATE24 | GhA12G0971 | 12 | 1368 | 455 | 36.6 | 6.21 | 细胞膜Cell membrane | GhMATE25 | GhA12G2733 | 12 | 1431 | 476 | 42.8 | 8.28 | 细胞膜Cell membrane | GhMATE26 | GhA13G0797 | 13 | 1518 | 505 | 48.7 | 8.17 | 细胞膜Cell membrane | GhMATE27 | GhA13G1248 | 13 | 1455 | 484 | 48.0 | 6.18 | 细胞膜Cell membrane | GhMATE28 | GhD01G0136 | 1 | 1586 | 528 | 49.5 | 5.08 | 细胞膜Cell membrane | GhMATE29 | GhD01G1006 | 1 | 1452 | 483 | 49.5 | 8.89 | 细胞膜Cell membrane | GhMATE30 | GhD02G1336 | 2 | 1518 | 505 | 50.8 | 5.59 | 叶绿体Chloroplast | GhMATE31 | GhD02G1620 | 2 | 1563 | 520 | 48.1 | 9.00 | 细胞质Cytoplasm | GhMATE32 | GhD02G1621 | 2 | 1545 | 514 | 48.4 | 7.66 | 细胞膜Cell membrane | GhMATE33 | GhD02G1761 | 2 | 1449 | 482 | 47.2 | 8.38 | 细胞膜Cell membrane | GhMATE34 | GhD02G1762 | 2 | 1470 | 489 | 47.0 | 7.96 | 细胞膜Cell membrane | GhMATE35 | GhD05G1622 | 5 | 1557 | 518 | 47.4 | 5.98 | 细胞质Cytoplasm | GhMATE36 | GhD05G2137 | 5 | 1476 | 491 | 47.8 | 8.34 | 叶绿体Chloroplast | GhMATE37 | GhD05G3073 | 5 | 1488 | 495 | 45.9 | 7.05 | 细胞膜Cell membrane | GhMATE38 | GhD05G3385 | 5 | 1416 | 471 | 44.2 | 8.28 | 细胞膜Cell membrane | GhMATE39 | GhD06G0281 | 6 | 1479 | 492 | 52.6 | 5.17 | 细胞膜Cell membrane | 基因 Gene | 序列号 Sequence number | 染色体 Chr. | 编码序列 Coding sequence (bp) | 蛋白残基数 Protein length (aa) | 分子量 Molecular weight (kD) | 等电点 pI | 亚细胞定位预测 Subcellular location prediction | GhMATE40 | GhD08G2334 | 8 | 1476 | 491 | 43.8 | 8.39 | 细胞膜Cell membrane | GhMATE41 | GhD09G0426 | 9 | 1476 | 491 | 49.9 | 5.75 | 细胞膜Cell membrane | GhMATE42 | GhD10G0979 | 10 | 1479 | 492 | 48.5 | 8.82 | 细胞膜Cell membrane | GhMATE43 | GhD11G0038 | 11 | 1533 | 510 | 48.5 | 8.65 | 细胞膜Cell membrane | GhMATE44 | GhD11G2135 | 11 | 1473 | 490 | 49.6 | 8.68 | 细胞质Cytoplasm | GhMATE45 | GhD11G2314 | 11 | 1452 | 483 | 48.7 | 8.57 | 细胞膜Cell membrane | GhMATE46 | GhD11G2334 | 11 | 1464 | 487 | 43.8 | 8.39 | 液泡膜Tonoplast | GhMATE47 | GhD12G0830 | 12 | 1521 | 506 | 53.9 | 6.99 | 细胞膜Cell membrane | GhMATE48 | GhD12G1083 | 12 | 1530 | 509 | 48.6 | 5.47 | 细胞膜Cell membrane | GhMATE49 | GhD13G1038 | 13 | 1377 | 548 | 41.9 | 7.61 | 细胞膜Cell membrane | GhMATE50 | GhD13G2211 | 13 | 1434 | 477 | 47.2 | 9.22 | 细胞膜Cell membrane | GhMATE51 | GhD07G0751 | 7 | 1314 | 434 | 47.8 | 7.49 | 细胞膜Cell membrane | GhMATE52 | GhD01G0461 | 1 | 1455 | 484 | 53.0 | 5.83 | 细胞膜Cell membrane | GhMATE53 | GhA01G0098 | 1 | 1125 | 374 | 40.6 | 8.94 | 细胞膜Cell membrane | GhMATE54 | GhA12G0513 | 12 | 1416 | 471 | 51.8 | 7.83 | 液泡膜Tonoplast | GhMATE55 | GhA07G0673 | 7 | 1314 | 437 | 47.4 | 5.33 | 细胞膜Cell membrane | GhMATE56 | GhA05G2769 | 5 | 1452 | 483 | 52.7 | 6.59 | 细胞膜Cell membrane | GhMATE57 | GhD03G1484 | 3 | 1563 | 520 | 56.6 | 8.46 | 液泡膜Tonoplast | GhMATE58 | GhA08G1613 | 8 | 1608 | 535 | 58.3 | 8.08 | 细胞膜Cell membrane | GhMATE59 | GhA12G2353 | 12 | 1644 | 547 | 59.7 | 6.69 | 细胞膜Cell membrane | GhMATE60 | GhA13G1249 | 13 | 1293 | 430 | 46.8 | 9.08 | 细胞膜Cell membrane | GhMATE61 | GhD07G0089 | 7 | 1512 | 503 | 55.1 | 6.59 | 细胞膜Cell membrane | GhMATE62 | GhA06G0589 | 6 | 1575 | 524 | 56.8 | 8.62 | 细胞膜Cell membrane | GhMATE63 | GhD09G1100 | 9 | 1506 | 507 | 54.0 | 5.89 | 细胞质Cytoplasm | GhMATE64 | GhA10G2322 | 10 | 1401 | 466 | 50.7 | 8.19 | 细胞膜Cell membrane | GhMATE65 | GhD09G2422 | 9 | 1311 | 436 | 47.4 | 7.05 | 细胞膜Cell membrane | GhMATE66 | GhA03G0114 | 3 | 1500 | 499 | 54.3 | 9.02 | 细胞膜Cell membrane | GhMATE67 | GhD02G0545 | 2 | 1482 | 493 | 53.2 | 7.94 | 叶绿体Chloroplast | GhMATE68 | GhA10G1472 | 10 | 1398 | 465 | 50.1 | 7.48 | 细胞膜Cell membrane | GhMATE69 | GhA03G2015 | 3 | 1563 | 520 | 56.5 | 8.21 | 细胞膜Cell membrane | GhMATE70 | GhA03G1320 | 3 | 1341 | 446 | 49.1 | 8.04 | 细胞膜Cell membrane | GhMATE71 | GhD05G2075 | 5 | 1662 | 553 | 60.3 | 7.47 | 细胞质Cytoplasm | GhMATE72 | GhD11G0087 | 11 | 1602 | 533 | 58.1 | 8.54 | 细胞膜Cell membrane | GhMATE73 | GhA09G0385 | 9 | 1581 | 526 | 57.8 | 8.70 | 细胞膜Cell membrane | GhMATE74 | GhA08G2165 | 8 | 1530 | 509 | 55.5 | 8.36 | 细胞膜Cell membrane | GhMATE75 | GhD06G0667 | 6 | 1575 | 524 | 57.0 | 8.58 | 细胞膜Cell membrane | GhMATE76 | GhA09G1094 | 9 | 1509 | 502 | 54.1 | 5.67 | 细胞膜Cell membrane | GhMATE77 | GhD05G0259 | 5 | 1617 | 538 | 58.4 | 7.51 | 细胞膜Cell membrane | GhMATE78 | GhD10G1146 | 10 | 1404 | 466 | 50.7 | 8.19 | 液泡膜Tonoplast | GhMATE79 | GhA02G0487 | 2 | 1524 | 507 | 54.8 | 7.48 | 细胞膜Cell membrane | GhMATE80 | GhA05G2384 | 5 | 1386 | 467 | 50.3 | 6.51 | 叶绿体Chloroplast | GhMATE81 | GhD12G2539 | 12 | 1119 | 392 | 42.6 | 5.48 | 细胞膜Cell membrane | GhMATE82 | GhD06G2314 | 6 | 1611 | 536 | 58.4 | 6.46 | 细胞膜Cell membrane | GhMATE83 | GhD12G2490 | 12 | 1632 | 543 | 59.2 | 8.90 | 细胞膜Cell membrane | 基因 Gene | 序列号 Sequence number | 染色体 Chr. | 编码序列 Coding sequence (bp) | 蛋白残基数 Protein length (aa) | 分子量 Molecular weight (kD) | 等电点 pI | 亚细胞定位预测 Subcellular location prediction | GhMATE84 | GhA11G0083 | 11 | 1602 | 533 | 58.1 | 8.34 | 细胞膜Cell membrane | GhMATE85 | GhD09G0403 | 9 | 1611 | 536 | 58.8 | 8.15 | 细胞膜Cell membrane | GhMATE86 | GhA09G2299 | 9 | 1404 | 467 | 50.7 | 7.09 | 细胞质Cytoplasm | GhMATE87 | GhA05G0185 | 5 | 1614 | 537 | 58.3 | 7.50 | 细胞膜Cell membrane | GhMATE88 | GhD03G1526 | 3 | 1500 | 499 | 54.0 | 8.85 | 叶绿体Chloroplast | GhMATE89 | GhD11G3461 | 11 | 1569 | 522 | 56.1 | 5.02 | 细胞膜Cell membrane | GhMATE90 | GhA03G0113 | 3 | 1500 | 424 | 45.7 | 8.40 | 细胞质Cytoplasm | GhMATE91 | GhD10G1714 | 10 | 1530 | 509 | 54.9 | 6.98 | 细胞膜Cell membrane |
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