基于甘蔗热带种(LA-purple)全基因组序列的SSR开发及特征分析

王恒波,肖乃衍,朱专为,刘翠翠,IntikhabALAM,陈平华,卢运海

Development and Characterization of SSR Markers from the Whole Genome Sequences of Saccharum officinarum (LA-purple)

Heng-Bo WANG,Nai-Yan XIAO,Zhuan-Wei ZHU,Cui-Cui LIU,ALAM Intikhab,Ping-Hua CHEN,Yun-Hai LU

图3 4对不同SSR引物在12个供试甘蔗属材料上的代表性PCR扩增图谱 1: 57NG208; 2: NG77-004; 3: 云南75-2-11; 4: 福建89-1-1; 5: 广东21; 6: 贵州78-2-28; 7: 黑车里本; 8: 路达士; 9: 克里斯塔林娜; 10: 拔地拉; 11: 桂糖35; 12: 科5; M: 100 bp DNA ladder。

Fig. 3 Representative PCR amplification patterns of four pairs of SSR primers of AT/TA motif in 12 tested Saccharum clones 1: 57NG208; 2: NG77-004; 3: YN75-2-11; 4: FJ89-1-1; 5: GD21; 6: GZ78-2-28; 7: Black cheribon; 8: Loethers; 9: Crystalina; 10: Badila; 11: GT35; 12: K5; M: 100 bp DNA ladder.